Figure S4 from Mass Spectrometry–Based Proteomics Identifies Serpin B9 as a Key Protein in Promoting Bone Metastases in Lung Cancer

Loss of SB9 can restore infiltration of CTLs. A-B: Quantification of tumour volume size by BLI curve in indicated groups.Collected tumor tissues one week after occurrence of BM and selected the certain volume of tumor tissues as same as possible. C: Flow cytometry analyses demonstrate that the infiltration of CD4+CD25+Foxp3+Tregs were no difference in BM from the SB9-silenced-group and SB9-overexpression-group. D-E: Flow cytometry analyses demonstrated that the infiltration of CD44highCD62LlowCD8+ effector memory T cells(D), and TNF-α+CD8+ T cells (E) were significantly higher in BM from the LLC1-Pa-sr-ctrl and LLC1-BM3-shSB9 group than the LLC1-Pa-OE-SB9 and LLC1-BM3-sr-ctrl group, respectively. F-G: Flow cytometry analyses show significantly lower percentages of pro-tumor-infiltrating IL-10 CD4+T cells(F) in LLC1-Pa-sr-ctrl and LLC1-BM3-shSB9 group than the LLC1-Pa-OE-SB9 and LLC1-BM3-sc-ctrl group, respectively. Moreover,anti-tumor-infiltrating IL-2 CD4+T(G) cells were significantly higher percentages in LLC1-Pa-sr-ctrl and LLC1-BM3-shSB9 group than the LLC1-Pa-OE-SB9 and LLC1-BM3-sr-ctrl group, respectively. H: Flow cytometry analyses demonstrated that the exhausted state(LAG-3) infiltration of CD8+T cells were no difference in BM from the SB9-silenced-group and SB9-overexpression-group. *P<0.05; **P<0.01; ***P<0.001; ns: not significant; P values were obtained by two-tailed unpaired t-test; The data are displayed as an average ± s.e.m.