Impact of varying doses of Moringa leaf extract supplementation in the cryopreservation media on sperm quality, antioxidant capacity and antimicrobial activity of frozen-thawed buck spermatozoa

The current investigation was planned to evaluate the effect of Moringa leaves also called Moringa oleifera (M. oleifera) aqueous extract on buck semen quality during cryopreservation, and its antimicrobial potential against the Escherichia coli (E. coli) isolated from the semen samples. Semen was collected from 8 Jakhrana bucks, and from each buck, 8 ejaculates were collected. Semen samples were subjected to bacteriological studies and pathogenic E. coli were isolated from semen samples. Then, various concentrations of M. oleifera extract were evaluated for its antimicrobial potential. Good quality semen samples were pooled during each collection. Pooled semen samples were then divided into 4 equal parts, and diluted in TRIS buffer containing different concentration of M. oleifera leaf aqueous extract (Different groups, i.e. T1-50 mg, T2-100 mg, T3-200 mg, C-0 mg of M. oleifera aqueous extract in 100 ml TRIS Buffer). Semen samples were evaluated for various sperm characteristics after cryopreservation. M. oleifera aqueous extract supplemented groups showed significant enhancement in sperm viability, sperm motility, acrosomal integrity and plasma membrane integrity. The treatment significantly reduced the lipid peroxidation in supplemented groups and M. oleifera extract shows the potent antimicrobial activity against the E. coli isolated from buck semen.