In-silico analysis for Structural and Functional Characterization of Phosphorus-Starvation Tolerance 1 (PSTOL1) Gene

As an important macro element for all living cells, phosphorus is essential in agricultural production systems and is required in large quantities by elite varieties of crops to maintain yields. Approximately 70% of the worldwide cultivated land suffers from phosphorous deficiency, and it has recently been estimated that the worldwide phosphorous resources will be shattered by the end of this century, thereby increasing the need to develop phosphorus-efficient crops. A greater understanding of how plants can maintain yield with lower phosphorous availability is highly desirable to both breeders and farmers. For this research, we focused on the phosphorusstarvation tolerance 1 (PSTOL1) gene, which is known to be involved in enhancing early root growth, thereby enabling plants to acquire more phosphorus and other nutrients and enhancing grain yield in phosphorus-deficient soil. As there is no reported structure and function of the PSTOL1 gene, this project involves a distinct set of opportunities and challenges and requires different approaches to model the interaction between PSTOL1 and the gene phosphorous uptake 1 (PUP1). This article covers the modeling, docking, and simulation of PSTOL1 to check the protein stability and its behavior over time. The physiochemical properties were ascertained, a phylogenetic tree was constructed to find the evolutionary relationship, and the conserved domains were analyzed for functional annotation. This study reports that the advancement of the PSTOL1-mediated phosphorous uptake 1 (PUP1) signaling cascade using structural bioinformatics is a potent biological mechanism against phosphorous starvation in wheat.