Effect of micro- and nanoplastic particles on human macrophages

Micro- and nanoplastics (MNPs) are ubiquitous in the environment, resulting in the uptake of MNPs by a variety of organisms, including humans, leading to particle-cell interaction. Human macrophages derived from THP-1 cell lines take up Polystyrene (PS), a widespread plastic. The question therefore arises whether primary human macrophages also take up PS micro- and nanobeads (MNBs) and how they react to this stimulation. Major aim of this study is to visualize this uptake and to validate the isolation of macrophages from peripheral blood mononuclear cells (PBMCs) to assess the impact of MNPs on human macrophages. Uptake of macrophages from THP-1 cell lines and PBMCs was examined by transmission electron microscopy (TEM), scanning electron microscopy and live cell imaging. In addition, the reaction of the macrophages was analyzed in terms of metabolic activity, cytotoxicity, production of reactive oxygen species (ROS) and macrophage polarization. This study is the first to visualize PS MNBs in primary human cells using TEM and live cell imaging. Metabolic activity was size- and concentration-dependent, necrosis and ROS were increased. The methods demonstrated in this study outline an approach to assess the influence of MNP exposure on human macrophages and help investigating the consequences of worldwide plastic pollution. Environmental implication Polystyrene micro- and nanoplastics are among the plastic particles which can be found worldwide in different ecosystems (1). Because of their size, they can enter the food chain and accumulate in a large variety of organisms (2). Previous studies already showed a cytotoxic effect on murine macrophages and human dTHP-1 (3, 4). However more research is needed to assess the impact of this distribution on humans. Studying the consequences of human plastic exposure and potential health risks using a human macrophage model, supports the process of political decision-making addressing plastic pollution.