MP26-04 HOW MIRABEGRON AFFECTS THE HUMAN URETER: AN IN VITRO STUDY

You have accessJournal of UrologyStone Disease: Medical & Dietary Therapy (MP26)1 May 2024MP26-04 HOW MIRABEGRON AFFECTS THE HUMAN URETER: AN IN VITRO STUDY Meylis Artykov, Esin Ozcelebi, Mehmet Yildirim Sara, Ahmet Gudeloglu, Alper Bektas Iskit, and Fazil Tuncay Aki Meylis ArtykovMeylis Artykov , Esin OzcelebiEsin Ozcelebi , Mehmet Yildirim SaraMehmet Yildirim Sara , Ahmet GudelogluAhmet Gudeloglu , Alper Bektas IskitAlper Bektas Iskit , and Fazil Tuncay AkiFazil Tuncay Aki View All Author Informationhttps://doi.org/10.1097/01.JU.0001009408.66023.77.04AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Aim of this study was to investigate the effects of mirabegron (MRB), a β3-adrenergic receptor agonist, on isolated human ureter. METHODS: The approval of the ethical committee was obtained before the study. This study was designed as series of in vitro experiments. Living donor graft kidney's ureters were preferred. Operations were between December 2020 and June 2021. Informed consent was obtained from all patients. Distal ureters were harvested at the back-table. Tissue was transferred in the flask with pre-gassed Krebs solution. Ureters were detubularized into stripes for organ baths. The tissue activity was recorded after stripes were left to rest until spontaneous contractions were observed. Consequently, responses were recorded by adding increasing concentrations of mirabegron. The activity was recorded via electro-mechanical transducer set-up using AcqKnowledge® 4.2 software (BIOPAC Systems, Inc.). Data analysis was performed with software SPSS 24.0 (IBM Corp., USA) and GraphPad Prism 6 (GraphPad, USA). RESULTS: 24 tissue strips from 16 ureters were prepared and studied. Only 15 strips showed spontaneous or inducible activity. The effect of MRB was investigated in tissues with spontaneous activity. The data of strips without activity was excluded. MRB lowered the frequency of spontaneous ureteric contraction in the concentration-dependent manner (Figure 1; A sample trace showing the effect of MRB in cumulative concentration on ureteral strips. Molar concentrations showed in means of logarithmic value). The decrease in frequency was statistically significant in presence of MRB at 10−6.5-10−4.5 M (Figure 2; Response values were compared with baseline contraction. All data in graphs is presented as arithmetic mean±standard error of the mean. a p<0.05, b p<0.005). Also, MRB reduced the spontaneous ureteric contractile force in a dose-dependent manner compared to the baseline contractile force. The decrease in amplitudes was statistically significant at 10−7-10−4.5 M MRB concentrations. CONCLUSIONS: MRB shows suppressive properties on the human ureter. This effect is achievable dose-dependently in isolated tissues. Download PPTDownload PPT Source of Funding: This study was financially supported by the Scientific Research Unit of Hacettepe University in Ankara Turkiye. © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e414 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Meylis Artykov More articles by this author Esin Ozcelebi More articles by this author Mehmet Yildirim Sara More articles by this author Ahmet Gudeloglu More articles by this author Alper Bektas Iskit More articles by this author Fazil Tuncay Aki More articles by this author Expand All Advertisement PDF downloadLoading ...