DNA barcoding reveals a new population of the threatened Atlantic Forest frog Sphaenorhynchus canga

Species identification plays a significant role in biodiversity conservation. As many species remain unrecognized, particularly in neotropical hotspots like the Brazilian Atlantic Forest (AF), novel molecular techniques are being widely employed to bridge this gap. In this study, we used DNA barcoding and phylogenetic tools to identify a new population of Sphaenorynchus canga in the central region of the Brazilian AF. Our results extend the species’ known distribution by approximately 200 km to the south, encompassing a different mountain range than its type locality (Serra do Espinhaço). This disjunct distribution, while not uncommon among amphibians, suggests a historical connection between these two mountain complexes as a biogeographic explanation. Despite the discovery of a new S. canga population, the species continues to face numerous anthropogenic threats such as mining, land use, and cattle ranching. Urgent conservation and research efforts are warranted to ensure the survival of S. canga populations across these habitats.