Three Undescribed Dihydrostilbene Glycosides from Leaves of Camellia Oleifera Abel. and Their Anti-Inflammatory Activity

Three previously unidentified dihydrostilbene glycosides, named oleiferaside A (1), oleiferaside B (2), and oleiferaside C (3), were discovered through a phytochemical exploration on Camellia oleifera Abel. leaves. Additionally, nine known secondary metabolites (4-12) were also identified. The undescribed secondary metabolites 1-3 were elucidated as 3,5-dimethoxydihydrostilbene 4′-O-α-L-arabinofuranosyl-(1→6)-β-D- glucopyranoside, 3,5-dimethoxydihydrostilbene 4′-O-α-L-arabinopyranosyl-(1→6)-β-D- glucopyranoside and 3,5-dimethoxydihydrostilbene 4′-O-β-D-apiofuranosyl-(1→6)-β-D- glucopyranoside, respectively. HR-MS and NMR spectroscopy were utilized for determining the structures of the isolates. The natural products were assessed for their anti-inflammatory effect using RAW264.7 macrophage stimulated by LPS. The findings demonstrated that compounds 1-4 exhibited inhibitory activities on NO and PGE2 production without causing cytotoxicity. These observations suggest that these compounds may have potential anti-inflammatory properties.