Identification of the Immune Repertoire of Γδ T-cell Receptors in Psoriasis

The gamma delta T cells are a subpopulation of T cells that are abundantly found in the skin and mucous membranes. Their reactivity to self-antigens and ability to secrete various cytokines make them a key component in psoriasis development. Although the correlation between the immune repertoire (IR) of gamma delta T-cell receptors and the occurrence and severity of psoriasis remains incompletely explored, high-throughput sequencing of gamma delta T cells has led to a deeper understanding of IR in psoriasis. This study investigated the differences between gamma delta T cells in patients with psoriasis and healthy controls. The gamma delta T cells were identified via immunofluorescence staining and a correlation analysis was performed according to the psoriasis area and severity index (PASI) scores. The IR sequencing method was used to detect IR in the gamma delta T-cell receptors. The findings demonstrated more skin gamma delta T cells in patients with psoriasis, which were positively correlated with the PASI score. There were subtle differences in most variable (V), diversity (D) and joining (J) gene segments and VJ/VDJ combination segments between patients with psoriasis and healthy controls. However, a higher diversity of complementarity-determining region 3 (CDR3) was observed in patients with psoriasis. In summary, the IR of skin gamma delta T cells was significantly altered in patients with psoriasis, and the diversity in the cell's CDR3 population is a promising biomarker for assessment of psoriasis severity. In this study, we found that the number of gamma delta T cells is directly proportional to the severity of psoriasis. The complementarity-determining region 3 diversity of gamma delta T-cell receptors in psoriatic lesion areas was much higher than that of healthy controls.ab image