谷歌浏览器插件
订阅小程序
在清言上使用

Microfluidic Platforms to Screen Granular Hydrogel Microenvironments for Tissue Regeneration

Lisa A. Krattiger,Dilara Borte Emiroglu, Silvia Pravato,Lukas O. Moser, Olivia A. Bachmann, Simona Y. La Cioppa, Gabriel J. Rodriguez Rivera,Jason A. Burdick,Andrew J. DeMello,Mark W. Tibbitt,Martin Ehrbar

ADVANCED FUNCTIONAL MATERIALS(2024)

引用 0|浏览16
暂无评分
摘要
AbstractGranular hydrogels have emerged as a promising class of biomaterials in medical research, enabling independent control of matrix stiffness within a porous biomaterial. Such microgel packings comprise interconnected pores and high surface‐to‐volume ratios. These features facilitate cell viability and nutrient diffusion, which are critical in enabling tissue regeneration. Despite the current interest in granular hydrogels for tissue engineering applications, only a few in vitro platforms are used to investigate cell interactions, limiting their design, and translation. In this study, microfluidic platforms able to reproducibly confine and immobilize microgels without the need for secondary cross‐linking are developed. Protocols are established for the generation of human bone marrow‐derived mesenchymal stem/stromal cells (hBM‐MSC)‐infiltrated microporous substrates and early‐time responses of cells to their environment are studied. Further, a tissue invasion assay is established, where cells infiltrate granular materials at different rates depending on growth factor presence or material properties. This platform is compatible with a range of different granular materials, and it is envisioned to have significant utility as a pre‐clinical tool for the rational design of materials for tissue healing applications.
更多
查看译文
关键词
biomaterial,cell-material interactions,granular hydrogel,microfluidics,microgels,platforms,porosity,regeneration
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要