CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations

Xiao-Wen Fan, Zi-Fan Gao, Dong-Dong Ling, De-Hui Wang, Ying Cui, Hui-Qun Du,Chun-Lin Li,Xing Zhou

MILITARY MEDICAL RESEARCH(2024)

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关键词
Cas9 nickase,CRISPR-Cas12a,Polymerase,Single base mutations
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