Structural characteristics and stability analysis of coconut oil body and its application for loading -carotene

Yan Tian, Xinxin Zhao, Zhiguo Wang,Weimin Zhang,Zhiguo Jiang

FOOD CHEMISTRY(2024)

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摘要
In this work, we investigated structural characteristics and stability analysis of the coconut oil body (COB) and its application for loading beta-carotene (beta-CA). The COB contained neutral lipids (81.1 +/- 2.1 %), membrane proteins (0.6 +/- 0.0 %), and moistures (18.3 +/- 3.2 %), in which the molecular weights of membrane proteins ranged from 12 kDa to 40 kDa, as analyzed by the SDS-PAGE. The COB exhibited a small droplet diameter (5.1 +/- 0.3 mu m) with a monomodal diameter distribution, as reflected by the dynamic light scattering. The COB showed stable states at alkaline pH values (pH 8-10) and instability against ionic strengths (50-200 mmol/L) and thermal treatment (30-90degree celsius) after analyzing the instability indexes. COB-based emulsions were favorable for the loading and retention of beta-CA, as reflected by free fatty acids release rates and bioaccessibility in the simulated gastrointestinal digestion. This study will contribute to using the coconut oil bodies for loading bioactive nutraceuticals to enhance their bioaccessibility.
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关键词
Coconut oil body,Loading beta-carotene,Composition,Physical stability,Free fatty acids release rates,Bioaccessibility
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