Cell-Free Adipose Tissue Extracts: A Novel Approach to Downregulate TRPV1 for Rosacea Treatment

Abstract Rosacea is a chronic inflammatory skin disease that typically affects the central facial area. Its main clinical symptoms include paroxysmal flushing, telangiectasia, and non-temporary erythema. Cell-free adipose tissue extracts (ATEs) are liquid components extracted from human adipose tissue that contain large amounts of growth factors. Despite the scar-reducing, anti-aging, and wound-healing effects of ATEs, the efficacy of ATEs in rosacea remains unknown. Therefore, the anti-rosacea effects of ATEs were investigated in LL37 cathelicidin-induced rosacea mice and capsaicin (CAP)-stimulated HaCaT keratinocytes. The in vitro results revealed that ATEs alleviated rosacea symptoms, such as erythema score, erythema area, transepidermal water loss, epidermal thickness, and telangiectasia upon downregulating TRPV1 and CD31 expression. Moreover, the up-regulated TRPV1 protein expression was also recovered by ATEs administration in vivo and in vitro. Meanwhile, ATEs demonstrated good biocompatibility. In summary, ATEs could be a potential therapeutic agent for rosacea by regulating inflammation and alleviating telangiectasia.