Electrochemical biosensor based on the integration of maple leaf-like gold nanocrystal and truncated aptamer for detection of α-amanitin with high sensitivity, selectivity and rapidity

This study reports the fabrication of three-dimensional gold nanocrystals as sensing material in the presence of l-glutathion and high-performance aptamer with 20 bases of α-amanitin via truncation and optimization of along aptamer. The resulting maple leaf–like gold nanocrystal (ML-Au) exhibits an improved catalytic activity due to more exposed high-index facets. The use of truncated aptamer increases the sensitivity by 15 times and reduces the reaction time by two times compared with those of original aptamer. An α-amanitin electrochemical biosensor constructed by integrating ML-Au nanocrystals with truncated aptamer exhibits high sensitivity, selectivity and rapidity. An increase of the α-amanitin concentration in the range of 1 × 10−14–1 × 10−9 M causes a linear decrease in the amperometric current with a limit of detection of 2.9 × 10−15 M (S/N = 3). The proposed analytical method is satisfactorily used for electrochemical sensing of α-amanitin in urine and wild mushroom samples.