A Multi-Year Longitudinal Harmonization of Quality Controls in Mass Spectrometry Proteomics Core Facilities

Quality control procedures play a pivotal role in ensuring the reliability and consistency of data generated in mass spectrometry-based proteomics laboratories. However, the lack of standardized quality control practices across laboratories poses challenges for data comparability and reproducibility. In response, we conducted a harmonization study within proteomics laboratories of the Core for Life alliance with the aim to establish a common quality control framework, which facilitates comprehensive quality assessment and identification of potential sources of performance drift. Through collaborative efforts, we developed a consensus quality control standard for longitudinal assessment and we adopted a common processing software. We generated a 4-year longitudinal dataset from multiple instruments and laboratories, which enabled us to assess intra- and inter-laboratory variability, to identify causes of performance drift, and to establish community reference values for several quality control parameters. Our study enhances data comparability and reliability and fosters a culture of collaboration and continuous improvement within the proteomics community to ensure the integrity of proteomics data.### Competing Interest StatementThe authors have declared no competing interest.* LC-MS : Liquid chromatography coupled to mass spectrometry HCD : Higher-energy collisional dissociation ETD : Electron-transfer dissociation EThcD : Hybrid electron-transfer/higher-energy collision dissociation CID : collision-induced dissociation