齿科技术、医疗器械专业领域内的技术开发、技术转让、技术咨询、技术服务，医疗器械生产（限分支机构经营），从事货物与技术的进出口业务，仓储（除危险化学品），自有设备租赁，软件的开发和销售，医疗器械、消毒产品、日用化学品、日用百货、电子产品、电器产品的销售，电子商务。【依法须经批准的项目，经相关部门批准后方可开展经营活动】
Objective The repair of bone defects caused by periodontal diseases is an arduous challenge in clinical treatment. ETV2 transcription factors belong to the E26 transformation specific（ETS） family. It is reported that ETV2 signaling molecules can play a key role in promoting angiogenesis and are ideal biological signaling molecules for periodontal tissue regeneration. However, the role of ETV2 in the osteogenesis of human dental pulp stem cells（hDPSCs） remains unclear and needs to be verified. Methods The lentivirus vector based on the dox induction system was used to realize the transgene overexpression of ETV2. Real time quantitative polymerase chain reaction（qRT-PCR）, western blot, immunofluorescence staining, alkaline phosphatase（ALP） staining and alizarin red S（ARS） staining were used to evaluate the effect of dox induced overexpression of ETV2 on hDPSCs osteogenesis. In addition, we also analyzed the potential mechanism of Etv2 induced osteogenesis by RNA sequencing（RNA Seq）. Results The experiment proved that ETV2 significantly overexpression up-regulated the osteogenic markers ALP, COL1A1 and OSX, significantly enhanced the activity of ALP, and promoted matrix mineralization hDPSCs. In addition, RNA sequencing and Western blot analysis showed that ETV2 was activated after the ERK/MAPK and PI3K-Akt signaling pathways were transduced. The overexpression of ETV2 can enhance the osteogenic differentiation of hDPSCs, which is partially reversed by the treatment of ERK/MAPK and PI3KAKT signal inhibitors. Conclusion The experimental results show that the overexpression of ETV2 has osteogenic effect on hDPSCs, which provides a new idea for periodontal tissue reconstruction.
