牙龈瘤是一种常见的牙龈增生性疾病,通常发生在龈乳头. 如瘤体组织较大,切除后可能会出现角化龈变窄以及骨面和根面暴露等不良预后情况. 牙龈瘤是一种在牙龈局部生长的炎性反应性瘤样增生物,常发生在牙龈乳头处[1] . 主要病因为菌斑、牙石等外界刺激.
Objective:To explore the mechanism of SIRT7 gene on autophagy protein and macrophage polarization in oral squamous cell carcinoma cells based on the NF-κB signaling pathway.Methods:Human oral squamous cell carcinoma cell line SCC25 cells were divided into SCC25 group(SCC25 cells without intervention),negative control group(SCC25 cells transfected with empty vector pLKO.1-vector-GFP),SIRT7 shRNA group(SCC25 cells transfected with SIRT7 shRNA),SIRT7 group(SCC25 cells transfected SIRT7 lentivirus),combination group A(SCC25 cells +50 μmol/L NF-κB inhibitor PDTC+ SIRT7 shRNA)and combination group B(SCC25 cells +50 μmol/L NF-κB inhibitor PDTC+SIRT7 lentivirus),the relative expression of SIRT7 was detected by qRT-PCR.The number of invasion was detected by Transwell method.The scratch heal-ing rate was detected by scratch test.Immunofluorescence detection of P62,LC3-Ⅱ,the levels of M1 and M2 marker proteins,NF-κB and NF-κB p65 proteins were detected by Western blot.Results:There was no significant difference in SCC25 cells invasion number,scratch healing rate,P62,LC3-Ⅱ,CD163,CD11C,NF-κB and NF-κB p65 expression between SCC25 group and negative control group(P>0.05).The number of cell invasion,scratch healing rate,CD163,NF-κB and NF-κB p65 were increased and CD11C was decreased in SIRT7 shRNA group(P<0.05).The invasion number,scratch healing rate,CD163,NF-κB and NF-κB p65 of SCC25 cells in SIRT7 group were decreased,while CD11C was increased(P<0.05).Compared with combination group A,the invasion number,scratch healing rate,CD163,NF-κB and NF-κB p65 of SCC25 cells in com-bination group B were decreased,and CD11C was increased(P<0.05).Conclusion:Up-regulation of SIRT7 can inhibit the invasion and migration of oral squamous cell cancer cells,reduce the activities of autophagy protein P62 and LC3-Ⅱ,and pro-mote the polarization of macrophage M2 to M1.The mechanism may be related to the regulation of NF-κB by SIRT7.
铁死亡是区别于凋亡、坏死、焦亡等细胞死亡方式的胞内铁依赖程序性细胞死亡方式,研究显示其参与神经系统、心血管系统、消化系统、免疫系统等相关疾病的发生、发展.近年来,谷胱甘肽超氧化物酶-谷胱甘肽-(半)胱氨酸[GPX4-GSH-Cyst(e)ine]途径介导的铁死亡在口腔疾病中也有报道.本文就铁死亡在口腔颌面部疾病中的研究进展及其作用机制作一综述,以期为铁死亡相关口腔疾病的防治提供新思路.
Objective:To analyse the differences in quality of life between patients with genuine halitosis and pseud-ohalitosis and healthy people.Methods:In this case-control study,178 subjects were selected and divided into 64 cases in the genuine halitosis group,60 cases in the pseudohalitosis group and 54 cases in the control group according to the halitosis com-plaints and detection results.A quality of life survey was carried out on these three groups by the 36-Item Short Form Health Survey(SF-36).Results:The genuine halitosis group and pseudohalitosis group scored lower than the control group in the three dimensions of physical functioning,validity and mental health(all P<0.05).Conclusion:Patients with halitosis experi-ence a significant reduction in quality of life in terms of validity and mental health compared to the normal population.
当受损的牙齿需要拔除时,意向性再植术是临床医生在日常实践中的重要治疗选择之一.其中,根尖的良好密封以抵御外界微生物是至关重要的.生物陶瓷材料因其优良的密封性和生物相容性被作为适宜的根尖倒充填材料,有利于提高意向性再植术的成功率.本文就生物陶瓷材料在意向性再植术中的应用现状及相关研究进展进行综述.
上颌后牙区拔牙后牙槽骨吸收和上颌窦气化等一系列生理变化常导致拟种植位点上颌窦底剩余骨高度不足,这为种植体植入带来了挑战[1] . 根据上颌窦解剖形态、手术风险等因素可选择侧壁开窗、经牙槽嵴顶等不同术式进行上颌窦底提升. 然而,单纯进行上颌窦底提升无法修复牙槽骨宽度、垂直向高度和患者的咬合关系,引起一系列生物及机械并发症.
唾液腺放射损伤作为头颈部放射治疗最常见的并发症,给患者的生活质量和口腔健康带来严重影响.目前针对唾液腺放射损伤的治疗局限于症状缓解.因此,寻找一种可行且高效的治疗方法尤为重要.干细胞治疗作为一种新兴的治疗策略,在放射损伤修复领域被认为通过促进受损组织的再生和修复,从而改善唾液腺放射损伤的症状和功能障碍.本文旨在系统地总结干细胞治疗在唾液腺放射损伤中的研究进展,并探讨其应用的安全性及有效性.
Objective:To analyze the effect of different filling methods of iRoot BP Plus on the sealing performance of root canal during apical surgery in vitro.Methods:Sixty freshly extracted mandibular first premolars meeting the inclusion criteria were randomly divided into three groups A,B and C with 20 teeth in each group.Group A underwent apicectomy after iRoot BP Plus positive filling,group B underwent apicectomy and iRoot BP Plus backfilling after conventional root canal ther-apy,and group C underwent apicectomy after conventional root canal therapy without root canal backfilling.The sealing prop-erty of the root canal of the three groups was analyzed qualitatively and quantitatively by dye leakage method.The adhesion and structural characteristics of the interface between the material and the root canal were observed by scanning electron mi-croscopy.Results:The three groups all had different degrees of dye penetration,and the average length of dye penetration was different(P<0.05).The average length of dye penetration in groups A and B was shorter than that in group C(P<0.05),but there was no significant difference in the average length of dye penetration in groups A and B(P>0.05).Scanning electron microscopy showed that iRoot BP Plus and dentin in groups A and B binded tightly and there were obvious cracks in group C.Conclusion:Both iRoot BP Plus materials with apicectomy after positive filling and iRoot BP Plus backfilling after conven-tional root canal treatment can be closely combined with dentin,and the apicectomy effect of both materials is similar.There-fore,apicectomy after iRoot BP Plus positive filling has certain clinical application value.
Objective:To construct a continuous nursing program for adolescent fixed orthodontic patients and to pro-vide guidance for the development of continuous nursing practice for adolescent fixed orthodontic patients.Methods:Based on the literature review and demand survey,two rounds of questionnaires were sent to 16 experts from the field of dental medicine and nursing using the Delphi method.Results:The expert active attitude coefficient of the two-round Delphi methods were 100%.The expert authority coefficient were above 0.8.The resulting continuous nursing program consists of five implementa-tion phases.Each phase contains four dimensions of implementation time,personnel,methods,and content.There were 18 im-plementation contents and a total of 60 details for implementation content.Conclusion:Based on the five treatment stages of fixed orthodontics,this study established a patient-centered,demand-oriented,comprehensive,refined,and actionable continu-ous care program,which established specific implementation time,implementation personnel,implementation methods and im-plementation contents,and was scientific and clinically practical.
牙源性透明细胞癌(clear cell odontogenic carcinoma,CCOC)是一种罕见的牙源性肿瘤,主要由空泡状或透明细胞组成. 根据世界卫生组织(WHO)1992 年对牙源性肿瘤的分类,CCOC被归类为具有局部侵袭能力的良性肿瘤. 然而,由于CCOC的局部破坏性和侵袭性行为,包括局部复发、区域淋巴结转移和罕见的远处转移,WHO在 2005 年将CCOC重新归类为"牙源性恶性肿瘤" .
Objective:To investigate the efficacy of photodynamic metal-organic framework PCN in killing Enterococ-cus faecalis in vitro.Methods:PCN was characterized by scanning electron microscopy(SEM)and full-wavelength enzymo-graph.The biosafety of PCN was verified by cell viability assay.The antibacterial properties of PCN in vitro were verified by gradient dilution counting.Results:PCN nanoparticles have strong ability of generating active oxygen and good biosafety.In vitro experiments showed that the concentration of 50 μg/mL could completely kill Enterococcus faecalis at the order of 108 CFU/mL,which had excellent antibacterial properties in vitro.Conclusion:This study verified the efficacy of photody-namic metal-organic framework PCN in killing Enterococcus faecalis,providing a new way for clinical root canal infection control.
Objective:To investigate the incidence and risk factors of postoperative delirium(POD)after in patients with head and neck cancer(HNC)and explore the correlation between prognostic nutritional index(PNI)and systemic im-mune-inflammation index(SII)with POD in head and neck cancer patients.Methods:A retrospective analysis of 206 patients in who were performed the radical resection with cervical lymph node dissection for HNC in the Xuzhou Central Hospital be-tween January 2017 to May 2023.Of the 206 patients,126 were male and 80 were female,aged 65～79 years with a mean of(60.80±12.51)years.Analysis of POD-related risk factors with SPSS 26.0 software package,and Logistic regression was used to screen for independent risk factors.Results:A total of 29(14.1%)patients had postoperative delirium.Single factor analysis results indicated that delirium was associated with age≥60 years old,male,tumor site,smoking,PNI,SII,total pro-tein,tracheostomy,blood transfusion,free flap reconstruction,time of operation,postoperative fever,sleep disturbance,ICU monitoring time,pneumonia,flap crisis,visual analogue scale/scores(VAS),length of hospital stay.Multiple logistic regres-sion analysis showed that the independent risk factors of delirium included age≥60 years old(OR=6.381,95%CI 1.218～33.421),PNI(OR=0.793,95%CI 0.649～0.970),SII(OR=1.002,95%CI 1.000～1.003),transfusion(OR=7.551,95%CI 1.180～48.320),sleep disturbance(OR= 7.411,95%CI 1.067～51.482),ICU monitoring time(OR= 3.879,95%CI 1.399～10.753),VAS scores(OR=1.648,95%CI 1.016～2.674).Conclusion:PNI and SII have good predictive values for POD in head and neck cancer patients,and it can be used to screen high-risk patients of POD.They have the potential to be-come a new routine tool for identifying patients who at high risk of POD.
Objective:To compare the difference of implant restorations made by partial intraoral scanning digital im-pressions and conventional impressions.Methods:Eighty-six patients requiring first molar implant restoration from June 2021 to December 2021 were randomly divided into 2 groups according to the mode of impression taking.Implant impressions were made using the partial intraoral scanning digital impression technique(group A)and the traditional silicone rubber impression technique(group B),respectively,from which the zirconia all-ceramic restorations were fabricated.Impression time and ad-justing time in the clinic were recorded and Geomagic software were used to calculate the amount of restorations adjustment.Patient's satisfaction was also investigated using the visual analogue scale(VAS).Results:There was statistically significant difference in the clinical adjusting time between the 2 groups of impressions(P<0.05).The marginal fit of the restorations in group A was significantly better than in group B(P<0.05).There was a statistically significant difference in the amount of occlusal surface sharpening in group A compared with group B(P<0.05).The patients'preference for digital impressions(group A)were higher,while the VAS scores of satisfaction with the restorations in the 2 groups were not statistically differ-ent.Conclusion:In the fabrication of single crowns of post-implant teeth,the use of local intraoral scanning digital impression technology not only saves clinical operation time and produces more accurate restorations with higher patient satisfaction than traditional impressions,but also achieves similar accuracy as traditional silicone rubber impressions.
修复体穿龈轮廓的设计对维持种植体周围软组织的健康具有重要影响.目前,修复体穿龈轮廓的形态、穿龈角度与种植体周炎的关系相关研究较少,且观点未能达成一致.种植体周炎的影响因素多参考天然牙,但因种植体与天然牙的结构大不相同,影响因素也不完全一致.本文将就穿龈轮廓在种植体周炎中的影响进行一综述,以此为临床医师提供参考.
目的:通过前瞻性研究分析评价附着龈缺失的种植患者在埋入愈合、穿龈愈合取模前行游离龈移植术的临床效果.方法:选取18例下后牙种植区因角化龈不足而行游离龈移植术的患者.种植术后2月角化龈宽度≤2 mm的患者(A组,埋入愈合);种植术后2个月颊侧角化龈宽度≤0.1 mm的患者(B组,穿龈愈合).比较游离龈移植术术后即刻,术后6周,戴牙当天和戴牙6个月颊侧角化龈垂直向宽度、宽度收缩率,及戴牙6个月种植体周围探诊深度(probing depth,PD)和牙龈探诊出血(bleeding on probing,BOP).结果:18例经游离龈移植的患者,术区龈瓣均成活.A组术后即刻角化龈宽度为(8.1±0.5)mm,术后6周(6.5±0.2)mm,戴牙当天(3.5±0.2)mm,戴牙6个月后平均宽度降为(3.1±0.2)mm;B组术后即刻为(7.1±0.1)mm,术后6周(5.9±0.1)mm,戴牙当天(5.1±0.1)mm,戴牙6个月后降为(4.9±0.1)mm;A组术后6周收缩率为(19.8±2.4)％,与B组收缩率(16.3+0.8)％差异有统计学意义;戴牙6个月比戴牙当天游离龈瓣收缩(11.2±3.5)％,较B组游离龈瓣收缩率[(8.8±1.0)％]大,但无显著性差异.戴牙6个月后两组间PD及BOP差异无统计学意义.结论:角化龈移植可有效增加附着龈宽度,且较之埋入种植的游离龈移植,穿龈愈合的游离龈移植术能获得更宽的角化龈和更小的补偿性收缩,但手术时机视种植体稳定情况而异.此外,龈瓣收缩亦存在个体差异.
目的:比较iRoot BP Plus和TheraCal LC对人牙髓细胞(human dental pulp cells,hDPCs)体外增殖分化的影响.方法:培养hDPCs,CCK-8法检测不同质量浓度iRoot BP Plus、TheraCal LC对hDPCs增殖的影响,确定最佳干预质量浓度.以最佳浓度培养hDPCs 7 d、14 d后,检测碱性磷酸酶(alkaline phosphatase,ALP)活性;培养2 d、4 d后检测成牙本质相关基因的表达.结果:iRoot BP Plus和TheraCal LC均能显著促进hDPCs的增殖、ALP活性以及成牙本质相关基因的表达,但两者之间并无明显统计学差异.结论:TheraCal LC促进hDPCs增殖及成牙本质分化的效果与iRoot BP Plus相当,值得临床推荐使用.
口腔鳞癌是最常见的口腔癌,但其治疗效果常不理想.口腔鳞癌的治疗失败多与肿瘤干细胞有关,该细胞亚群增殖能力强、侵袭性高,且具有自我更新和不断分化的特性,在口腔鳞癌的侵袭、转移和复发等各方面发挥着重要作用.在口腔鳞癌中,发生了肿瘤干细胞相关信号通路的异常激活,如Wnt/β-catenin、Sonic-Hedgehog、Notch和EGFR等通路,以上信号通路共同维持肿瘤干细胞发挥作用并调节口腔鳞癌发生发展.了解口腔鳞癌干细胞相关信号通路可有利于治疗口腔鳞癌,减少或预防其复发、转移.本文就肿瘤干细胞相关信号通路在口腔鳞癌中的研究进展进行阐述.
牙髓再生组织工程技术是基于生物学、材料学和工程学等多个学科的交叉研究,通过构建组织细胞-生物材料三维空间复合体,植入患牙髓腔,以促进无髓患牙的血运重建和组织细胞的增殖分化,并最终形成修复性牙髓-牙本质复合体.组织工程技术在牙髓再生的治疗中有巨大应用前景,有望取代根管治疗成为损伤牙髓修复的主流方法.本文对牙髓再生组织工程技术构建的仿生支架和体外模型进行了综述,以期对组织工程技术在牙髓再生中的作用有更深入的理解和新的启示.
目的:探究环状RNA(circRNA)信号诱导增殖相关蛋白 1 样蛋白 1(signal-induced proliferation-associat-ed 1 like 1,SIPA1L1)修饰人牙髓干细胞(human dental pulp stem cell,hDPSC)来源外泌体(exosome,Exo)对脂多糖(lipopolysaccharides,LPS)刺激下的 hDPSC 成骨分化能力的影响.方法:将 circSIPA1L1 过表达质粒载体转染至hDPSC后分离Exo,透射电镜观察形态特征,Western blot测定Exo标志蛋白CD81、Hsp70、TSG101 表达,qRT-PCR检测circSIPA1L1 相对表达量,用PHK26 荧光标记Exo并观察 hDPSC摄取情况.hDPSC分为对照组、LPS组、LPS+ hDPSC Exo组、LPS+circSIPA1L1-hDPSC Exo 组,CCK-8 检测各组 hDPSC 增殖;酶联免疫吸附测定(ELISA)各组hDPSC培养上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6 水平,茜素红染色检测各组hDPSC红色钙结节形成,Western blot测定各组hDPSC中碱性磷酸酶(ALP)、骨钙素(OCN)、骨桥素(OPN)、Runt相关转录因子 2(Runx2)及牙本质涎磷蛋白(DSPP)蛋白表达.结果:hDPSC与转染circSIPA1L1 后的hDPSC中分离得到椭圆形或圆形颗粒物,直径大多分布在约 100 nm处,CD81、Hsp70 及TSG101 蛋白表达明显,且circSIPA1L1 修饰hDPSC来源Exo中 circSIPA1L1 相对表达量显著高于 hDPSC 来源 Exo(P＜0.05),并观察到 hDPSC 来源 Exo 与 circRNA SIPA1L1 修饰hDPSC来源 Exo 均可被 hDPSC 所摄取.与 LPS 组比较,LPS+hDPSC Exo 组和 LPS+circSIPA1L1-hDPSC Exo组hDPSC增殖能力显著升高(P＜0.05),hDPSC 培养上清液中 TNF-α、IL-1β、IL-6 水平显著降低(P＜0.05),红色钙结节明显增多,细胞中ALP、OCN、OPN、Runx2、DSPP蛋白相对表达量显著上调(P＜0.05).此外,与LPS+hDPSC Exo组比较,LPS+circSIPA1L1-hDPSC Exo组hDPSC增殖能力显著升高(P＜0.05),hDPSC培养上清液中TNF-α、IL-1β、IL-6水平也显著降低(P＜0.05),红色钙结节更多,细胞中ALP、OCN、OPN、Runx2、DSPP蛋白相对表达量也显著上调(P＜0.05).结论:circRNA SIPA1L1 修饰hDPSC来源的Exo能够提高LPS刺激下hDPSC的增殖水平,抑制促炎因子释放,增强hDPSC的成骨分化能力.
目的:探究口腔正畸联合修复治疗在牙列缺损合并牙颌畸形患者中的应用效果.方法:回顾性分析我院 2021 年 7 月～2022 年 7 月期间收治的牙列缺损合并牙颌畸形患者 76 例,分为观察组和对照组,各 38 例.对照组采用常规修复治疗,观察组采用口腔正畸联合修复治疗.牙列缺损修复完成 12 个月后随访观察两组治疗效果,包括治疗优良率、不良反应发生情况、牙齿排列整齐率、前牙覆良好率、后牙咬合良好率.评估治疗前后吞咽功能、咀嚼功能、语言功能评分变化;采用咀嚼效率及咬合力对患者口腔功能进行评定,观察骨吸收量、恢复正常时间及牙齿美观评分情况.结果:牙列缺损修复完成 12 个月后,观察组治疗优良率高于对照组(P＜0.05).不良反应发生情况两组对比无差异(P＞0.05).观察组牙齿排列整齐率、前牙覆良好率、后牙咬合良好率均高于对照组(P＜0.05).治疗前,两组吞咽功能评分、咀嚼功能评分、语言功能评分对比无差异(P＞0.05);治疗后,观察组评分均优于对照组(P＜0.05).治疗前,两组咀嚼效率及咬合力情况对比无差异(P＞0.05);治疗后观察组咀嚼效率及咬合力情况均优于对照组(P＜0.05).观察组骨吸收量及恢复时间均少于对照组,牙齿美观评分高于对照组(P＜0.05).结论:口腔正畸联合修复治疗在牙列缺损合并牙颌畸形患者中具有显著效果,能够改善患者口腔功能及咬合能力,使其恢复正常咀嚼状态,保持患者颌面部美观性.
