American School of Classical Studies at Athens

The Middle Pleistocene (MP; ca. 774 to 129 ka) marks a critical period of human evolution, characterized by increasing behavioral complexity and the first unambiguous evidence of plant-based technologies. Despite this, direct evidence for early wooden tool use remains exceptionally rare. Here, we present the earliest handheld wooden tools, identified from secure contexts at the site of Marathousa 1, Greece, dated to ca. 430 ka (MIS12). Through a systematic morphological, microscopic, taphonomic, and taxonomic analysis of the sampled wood macroremains, two specimens were securely identified as modified by hominins: one small alder (Alnus sp.) trunk fragment bears clear working and use-wear traces consistent with a multifunctional stick likely used in digging at the paleolakeshore; and one very small willow/poplar (Salix sp./Populus sp.) artifact exhibits signs of shaping and potential use-wear. A third specimen, a large alder trunk segment, shows deep, nonanthropogenic striations interpreted here as claw marks from a large carnivoran. The wooden tools were excavated together with butchered elephant remains, small lithic artifacts and debitage, and worked bone, underscoring the diversity of engagement with a variety of different raw materials for technological purposes at Marathousa 1. These finds extend the temporal range of early wooden tools. They represent both the use of expedient larger handheld tools as well as a much smaller, likely finger-held wooden tool, which is uniquely small for the Pleistocene, expanding known functional purposes of early wood technologies. Moreover, they highlight the Megalopolis Basin's exceptional preservation conditions and its role in understanding the evolution of hominin behavior.

During the Last Glacial Maximum (LGM) 26–19 thousand years ago (kya), Europe had three principal refugia not covered by ice: the Iberian, Italian, and Balkan peninsulas 1,2 . While the Iberian and Italian refugia have been studied with ancient DNA 3–7 , the legacy of the Balkan refugium has remained a mystery due to a lack of genetic data 30 – 12 kya. We present genome-wide data from nine newly reported individuals: three Epipaleolithic from Romania, one Epipaleolithic from Bosnia and Herzegovina, three Mesolithic from Greece, one Epipaleolithic from Southern Italy, and one Mesolithic from Sardinia. We find that the Epipaleolithic individuals from Romania and the Mesolithic individual from mainland Greece were from a previously unsampled population that was the primary source for later European hunter-gatherers. Westward expansion of Balkan Epipaleolithic people into the Italian Peninsula and mixture with a minority contribution from pre-LGM Italians, produced a population that then spread further west to become the primary ancestry of west-ern European Mesolithic people. Northeastward expansion, bypassing Italy, contributed the European ancestry source of Scandinavian and Eastern European hunter-gatherers. Southeastward expansion to the Aegean led to Anatolian-European mixtures before the spread of farming.

This study investigates animal husbandry, fishing techniques, diet and their socio-economic implications within the changing political landscape of Late Byzantine Thebes (13th-15th centuries CE) through an integrated zooarchaeological approach. It draws evidence from faunal assemblages from 9 refuse pits (bothroi) on the Ismenion Hill, an area with some habitation in southeastern Thebes. Results indicate a reliance on caprines—especially sheep—as the dominant livestock, suggesting an organized system of mutton production. Pigs and cattle likely played a supplementary role in inhabitants’ diet and economic activities, at least in the examined households. A portion of the herds were likely in-house stalled while deadstock was processed in a standardized way. Fish remains indicate a marked reliance on aquatic resources from near-shore, coastal marine environments, with a notable abundance of the gilthead seabream (Sparus aurata). Furthermore, while Thebes experienced successive foreign rule from the 13th century onward, continuity in animal exploitation during these times suggests resilience in local foodways. Ismenion Hill residents matched the general trends in animal exploitation and meat consumption seen in other regions of the Greek peninsula since the first centuries of the Byzantine Period. These findings contribute significantly to the underexplored field of Byzantine zooarchaeology and shed light on subsistence strategies in medieval Greece.