The Pennsylvania Game Commission (PGC) is the state agency responsible for wildlife conservation and management in Pennsylvania in the United States. It was originally founded 127 years ago and currently utilizes more than 700 full-time employees and thousands of part-time and volunteers in its official mission to "manage and protect wildlife and their habitats while promoting hunting and trapping for current and future generations.
Much of the research into white-nose disease has focused on the hibernation period, while the pathogenic fungus Pseudogymnoascus destructans is actively infecting the bat host. Previous research has found large differences between the susceptible North American Myotis lucifugus and the tolerant European Myotis myotis, suggestive of immunopathology in the former, and a beneficial lack of strong response in the latter. Here, we examined gene expression in these species both during the late-hibernation period and a month after emergence from hibernation, during healing from infection. We utilised paired sampling, collecting wing tissue that was positive and negative for fungal infection fluorescence, to examine changes in wholetranscriptome gene expression that were local to sites of infection at two time points: pre-emergence and 30 days post-emergence from hibernation. Positive samples were contrasted between the two time points to examine longitudinal changes. During the pre-emergence period, local inflammatory responses were observed in both M. myotis and M. lucifugus. Immune responses between the tolerant and susceptible species were dissimilar, favouring Th1 and Th17 cytokine responses, respectively. This lends weight to immunopathology as a contributing factor to mortality in M. lucifugus. Continual immune responses may not only contribute to immunopathology and host mortality but also have important carry-over effects on reproduction and subsequent pre-winter fattening, affecting population viability over a longer period of time than previously considered.
Interactions between co-occurring pathogens can have complex and significant impacts on host survival, fitness, and population dynamics. While common in wildlife, coinfections are often overlooked, and research may create biased management perspectives when individual pathogens are assessed in isolation. Recent work has found that wild turkeys (Meleagris gallopavo) are affected by various pathogens, but it is unknown how infections and coinfections are spatially structured or interact with each other. Here, we determined the associations and risk factors of infection by lymphoproliferative disease virus (LPDV), reticuloendotheliosis virus (REV), three avian Mycoplasma species, and internal parasites in Pennsylvania wild turkeys. Our results indicate varying prevalences: LPDV (70%), REV (1%), Mycoplasma gallisepticum (0%), Mycoplasma meleagridis (4%), Mycoplasma synoviae (2%), and internal parasites (63%). The prevalence of LPDV was greater in adults than juveniles but did not vary with year, sex, study area, or landscape type. Parasite species richness was greater in juveniles than adults, greater in males than females, varied by year and study area, but did not vary with landscape type. Coinfections with LPDV and parasites were more common (41%) than infections with only LPDV (26%) or only parasites (22%). All other coinfection prevalences involving viruses, Mycoplasma species, and parasites were low (0%-3%). Finally, infection with LPDV did not differ with overall parasite species richness but was negatively associated with infection with parasitic nematodes. These results reveal high rates of coinfections with LPDV and parasites in turkeys but suggest that parasite infections are independent of LPDV infections. Ongoing work is currently investigating the sublethal effects of these coinfections on wild turkey populations.
Chronic wasting disease (CWD) is an always-fatal disease infecting wild cervids globally. Ecologically and economically important, CWD presents a challenge for managing white-tailed deer (Odocoileus virginianus). We built an agent-based model to simulate CWD transmission and assess potential management actions that could slow disease spread: PAOvCWD. We developed PAOvCWD using contact rates and other behavioral and ecological metrics estimated from deer monitored in Pennsylvania, USA. We programmed potential management responses (e.g., culling, altered hunter harvest) for all 22 Pennsylvania wildlife management units and validated the efficacy of PAOvCWD using a deer population in south-central Pennsylvania infected with CWD for > 10 years. To support applications of our model, we developed a user-friendly R pipeline that allows implementation with relatively minor modifications. Our pipeline includes four steps:•Steps 1 and 2 generate regional percent forest cover rasters and curate population-level demographics.•Step 3 initializes landscapes and agents using our PAOvPOP model.•Step 4 assesses CWD transmission and management responses using our PAOvCWD model.
Effective conservation of wildlife is often hindered by poor understanding of where focal species and their habitats occur across large landscapes. Advancements in remote sensing have enabled researchers to improve detection of focal species (e.g. autonomous recording units [ARUs]) and the characterization of their habitats (e.g. light detection and ranging [LiDAR]), thus mitigating these issues. Research into ruffed grouse Bonasa umbellus, a declining forest game bird, stands to benefit from these technologies given the species' low detectability and preference for particular forest structure conditions that are difficult to capture using imagery‐based remotely sensed data. Herein, we investigated regional occurrence of ruffed grouse across Pennsylvania via the use of a multi‐year passive acoustic monitoring dataset, fine scale LiDAR‐derived forest structure metrics, and a suite of other forest and landscape variables to predict state‐wide ruffed grouse occurrence probability and identify areas in need of targeted habitat management. Our analyses indicated that, well‐connected, high‐elevation hardwood forests with some conifers and well‐developed understories (e.g. timber harvests) were predicted to have the highest probability of grouse occurrence. Likewise, similar forests with open understories (e.g. mature forests within similar landscape contexts) were predicted to be the most promising for future management. ARUs proved to be effective at building a large detection dataset, which when paired with the superior forest structure data provided by LiDAR, allowed us make predictions about ruffed grouse across Pennsylvania with greater confidence than ever before.
Rabies is a universally fatal viral disease that affects a wide variety of mammalian species, including carnivores and herbivores. While testing and confirmed reports in wild carnivores are common, comparatively less is known about this disease in wild herbivores. Between 2022 and 2024, nine white-tailed deer (Odocoileus virginianus) in Pennsylvania, USA were diagnosed with rabies. Eight (89%) presented with erratic neurologic signs and one was found dead. Evidence of chronic self-mutilation characterized by hair loss and thickened, hardened, and hyperpigmented skin was present on the head in seven cases (78%) and/or body or legs in two cases (22%), while two (22%) did not have any evidence of external lesions. Microscopically, lesions were like those in other mammalian species, with variably severe inflammation and abundant cytoplasmic inclusion bodies with associated antigen immunoreactivity. This case series highlights a common and characteristic gross lesion that can prompt caution and testing for rabies in deer and further expands understanding of this disease in an uncommonly diagnosed species.
