Budidaya kerang merupakan salah satu sektor perikanan yang memiliki potensi ekonomi tinggi, khususnya di wilayah pesisir Indonesia seperti Bagansiapiapi. Salah satu faktor penentu keberhasilan budidaya adalah ketersediaan bibit kerang berkualitas. Namun, proses distribusi dan akses informasi mengenai bibit kerang di wilayah ini masih dilakukan secara konvensional, melalui perantara atau jaringan informal, yang seringkali menimbulkan risiko seperti penipuan, ketidaksesuaian produk, dan keterlambatan pengiriman. Belum tersedianya platform digital khusus juga menjadi kendala dalam menciptakan ekosistem jual beli  yang efisien dan terpercaya. Penelitian ini bertujuan merancang dan mengembangkan aplikasi marketplace bibit kerang berbasis Progressive Web App (PWA) dengan pendekatan User-Centered Design (UCD). PWA dipilih karena mampu diakses tanpa instalasi aplikasi, mendukung notifikasi, serta tetap berfungsi dalam kondisi konektivitas terbatas. Pendekatan UCD diterapkan agar perancangan antarmuka dan pengalaman pengguna sesuai dengan kebutuhan dan konteks pengguna lokal. Beberapa studi sebelumnya menunjukkan bahwa metode UCD dapat meningkatkan kualitas UI/UX dan efektivitas aplikasi, dibuktikan melalui skor System Usability Scale (SUS) yang tinggi. Aplikasi ini dilengkapi fitur pencarian bibit, sistem pembayaran aman (Pay Protection), ulasan pengguna, dan konsultasi dengan ahli (Aqua Expert). Dengan adanya solusi digital ini, diharapkan proses transaksi menjadi lebih aman, cepat, dan efisien, sekaligus mendukung produktivitas pembudidaya serta memperkuat transformasi digital di sektor perikanan lokal.
Introduction: Short chain fatty acids (SCFAs), such as acetic, propionic, and butyric acid, areproduced by fermentation of dietary fibers by the gut bacteria. Deregulation in production andhandling of SCFAs is associated with cardiovascular dysfunction in humans and rodents.However, limited studies have investigated the effect of SCFAs on cardiovascular parameters inthe zebrafish (ZF), an increasingly popular model in cardiovascular and gut microbiota research. Methods: Bacterial 16s sequencing and GC-MS were performed in adult male and female Tg(NFkB:EGFP) ZF fecal samples for analysis of the gut microbiota and SCFAs respectively. Inanaesthetized ZF larvae (7-9 dpf; MS-222, 0.3mM), measurements of heart rate (HR, bpm) andvascular tone (width of dorsal aorta, pixels) were performed every 10 minutes for an hour usinglive imaging (Nikon, 4x magnification) following administration of physiologic concentrations ofacetate (27.4 μM), butyrate (2.2 μM) and propionate (1.7 μM; pH=7.5) via immersion. Results: Relative proportions of SCFAs in the ZF were similar to those previously reported in rats andhumans, with highest levels of acetic acid (27.43 μM) followed by butyric (2.19 μM) and propionic(1.65 μM) acid. At the phylum level, the observed abundances of Proteobacteria, Firmicutes, andFusobacteria were similar to those reported in rodents and humans. Administration of selectSCFAs resulted in a ~20% decrease in HR at all time points following administration of butyrate(P<0.0001), and at 40-60min following administration of acetate (P<0.05), with no effect ofpropionate. In addition, a ~25% decrease in the width of the dorsal aorta was observed at 60 minfollowing administration of butyrate (P<0.05), with no effect of acetate or propionate. Conclusion: We report a similar composition and abundances of the gut microbiota and SCFAs between ZF,rodents and humans. In addition, both butyrate and acetate produced a bradycardia in the ZF,while the observed butyrate-dependent decrease in the vascular tone may be compensatory tobradycardia. Our findings demonstrate the potential for ZF as a model to study host-microbiotainteractions in regulation of cardiovascular function.
ENVIRONMENTAL SCIENCES Correction for “Genome sequence of the button mushroom Agaricus bisporus reveals mechanisms governing adaptation to a humic-rich ecological niche,” by Emmanuelle Morin, Annegret Kohler, Adam R. Baker, Marie Foulongne-Oriol, Vincent Lombard, Laszlo G. Nagy, Robin A. Ohm, Aleksandrina Patyshakuliyeva, Annick Brun, Andrea L. Aerts, Andrew M. Bailey, Christophe Billette, Pedro M. Coutinho, Greg Deakin, Harshavardhan Doddapaneni, Dimitrios Floudas, Jane Grimwood, Kristiina Hilden, Ursula Kues, Kurt M. LaButti, Alla Lapidus, Erika A. Lindquist, Susan M. Lucas, Claude Murat, Robert W. Riley, Asaf A. Salamov, Jeremy Schmutz, Venkataramanan Subramanian, Han A. B. Wosten, Jianping Xu, Daniel C. Eastwood, Gary D. Foster, Anton S. M. Sonnenberg, Dan Cullen, Ronald P. de Vries, Taina Lundell, David S. Hibbett, Bernard Henrissat, Kerry S. Burton, Richard W. Kerrigan, Michael P. Challen, Igor V. Grigoriev, and Francis Martin, which appeared in issue 43, October 23, 2012, of Proc Natl Acad Sci USA (109:17501–17506; first published October 8, 2012; 10.1073/pnas.1206847109). The authors note that the following statement should be added to the Acknowledgments: “G.D. is a PhD student funded by the Teagasc Walsh Fellowship scheme and jointly supervised by K.S.B., and HM Grogan, Teagasc, Ireland.”
