Collagenolytic proteases have attracted increasing attention due to their ability to hydrolyze collagen and their relevance in biotechnological processes. In this study, a collagenolytic protease produced by Mucor subtilissimus UCP 1262 was characterized through an integrated evaluation of its biochemical properties, thermal-inactivation thermodynamics, and conformational behavior. The enzyme was produced by solid-state fermentation using wheat bran and purified by DEAE-Sephadex A50 ion-exchange chromatography. Optimal collagenolytic activity was observed at pH 7 and 40 degrees C, and approximately 50% of the initial activity was retained after 24 h at 30-40 degrees C, indicating moderate thermal stability. Fluorescence spectroscopy revealed marked pH- and temperaturedependent changes in fluorescence intensity and emission maxima, with incomplete structural recovery after cooling. Thermodynamic analysis further supported the occurrence of temperature-dependent destabilization. Overall, the results provide new insight into the functional and conformational stability of the collagenolytic protease from M. subtilissimus UCP 1262 and support its further investigation for biotechnological applications under mild processing conditions.
Introduction: The new corona virus (2019-nCoV OR HCOV-19 or CoV2), has emerged in China as the main cause of viral pneumonia (COVID-19, Coronavirus Disease-19). Objective: To provide evidence-based Physiotherapy and functionality in patients with adult and pediatric COVID-19. Methods: This is an integrative literature review using the MedLine / PubMed databases, library of Latin American and Caribbean Literature in Health Sciences (LILACS) and Physiotherapy Evidence Database (PEDRo). Results: Part of the patients with covid 19 show signs of respiratory deficiency with hypoxemia, with low severity in children. Impaired functionality is also expected. Conclusion: COVID-19 causes low pulmonary compliance and important changes in lung function with hypoxemia and cardiovascular repercussions. These changes lead to the need for Physiotherapy and the management of oxygen therapy and ventilatory support (invasive and non-invasive) for these patients.
The aim of this study was to evaluate the association among anxiety, symptoms of temporomandibular disorders, and awake bruxism in children. A cross-sectional study was carried out with 274 children aged between 7 and 12 years. Data were collected through structured questionnaires, applied by interview and clinical assessment. Statistical analysis involved descriptive and inferential analysis using the Chi-square test and Fisher’s exact test. A binary logistic regression model was applied to identify independent predictors of anxiety. Age ranged from 7 to 12 years and was equally distributed between the sexes. 23.3
Parkinson’s disease (PD) is a progressive neurodegenerative disorder with no proven disease-modifying therapies to date. Because changes in cerebral glucose metabolism and insulin resistance have been linked to PD pathophysiology, glucagon-like peptide-1 receptor agonists (GLP-1RAs), widely used for diabetes, have been investigated as potential neuroprotective treatments. This study systematically assessed the efficacy and safety of GLP-1RAs in PD through a systematic review and meta-analysis of randomized controlled trials identified in PubMed, Embase, and the Cochrane Library. The primary outcomes were motor function improvements measured by the MDS-UPDRS Part III in both on- and off-medication states at study endpoints and at intermediate timepoints of interest. Secondary outcomes included MDS-UPDRS Parts I, II, and IV, quality of life assessed by the PDQ-39, levodopa equivalent daily dose (LEDD), and the occurrence of adverse events. The meta-analysis found no statistically significant difference in favor of GLP-1RAs over placebo for motors and non-motors outcomes, except for PDQ-39 (MD: − 0.75; 95
We consider a rational scalar field model in (1+1)-dimensions where the long-range character of the kinks is controllable. We show via numerical simulations that kinks with long-range tails on both sides can exhibit resonance windows. The resonant energy exchange mechanism occurs via the excitation of quasinormal modes, which we obtain via a spectral analysis. Additionally, we locate a resonance window in a family of ϕ ^10 models with long-range tails on both sides. Moreover, we propose a new algorithm for initializing long-range kink collisions, based on convection–diffusion dynamics.
