AllergyVolume 32, Issue 5 p. 301-303 DIURNAL VARIATION IN THE NUMBER OF CIRCULATING EOSINOPHIL LEUCOCYTES IN NORMAL CONTROLS AND ASTHMATICS RONALD DAHL, Corresponding Author RONALD DAHL From: Medical department II, Århus Amtssygehus, Denmark.Ronald Dahl Med. dept. II Århus Amtssygehus 8000 Århus C DenmarkSearch for more papers by this author RONALD DAHL, Corresponding Author RONALD DAHL From: Medical department II, Århus Amtssygehus, Denmark.Ronald Dahl Med. dept. II Århus Amtssygehus 8000 Århus C DenmarkSearch for more papers by this author First published: October 1977 https://doi.org/10.1111/j.1398-9995.1977.tb02571.xCitations: 25AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Citing Literature Volume32, Issue5October 1977Pages 301-303 RelatedInformation
An extract of mixed dog hair and dandruff from six different dog breeds (alsatian, boxer, collie, poodle, and long-haired and short-haired dachshund) was obtained by mild extraction, centrifugation, dialysis and freeze-drying. Extract of hair and dandruff from the individual dog breeds was obtained in the same way, but the material was not freeze-dried. Examination and characterization of the mixed extract by means of crossed immunoelectrophoresis revealed a precipitation pattern composed of 25 antigens, some of which were mutually partially identical, and a high content of dog serum proteins was found. Quantitative and qualitative differences between the individual dog breeds were demonstrated. Partial identity of the antigens of the mixed extract with antigens of serum, antigens of extracts of hair and dandruff from cat, cow, horse and guinea pig, and antigens from extract of house dust was also observed. By means of crossed radioimmunoelectrophoresis, using sera from 21 patients who were RAST-positive to dog hair and dandruff extract, the specific IgE-binding to antigens of the mixed extract was examined. On the basis of these results major and minor allergens were identified. Dog albumin was found to be a very important major allergen, but alpha1-antitrypsin and gamma-globulin were also identified. Furthermore, four non-serum proteins were shown to be allergens. No breed-specific allergens could be identified in the extracts from the individual dog breeds.
The effect of locally acting intra-nasal beclomethasone dipropionate was observed in 42 patients suffering from seasonal allergic rhinitis and 50 patients suffering from perennial rhinitis. The study consists of three parts: 1) a simple double-blind trial in seasonal rhinitis, 2) a double-blind crossover trial in perennial rhinitis, 3) a 1-year follow-up of the perennial group. The active drug was significantly superior to placebo when comparing subjective symptoms, nasal resistance and secretion eosinophilia in the patients. In long-term use it was possible to reduce the initial dose considerably. On objective examination no significant changes were seen in the epithelium following long-term use. No suppression of the hypothalamic-pituitary-adrenal axis was observed after 1 year's regular use of beclomethasone dipropionate. The side effects were mild, the most common complaint being irritation of the nasal mucous membrane, apparently caused by the propellant gas.
The effect of SCG powder (Lomudalr, Intalr) intranasally in vasomotor rhinitis has been studied on 49 adult volunteers in a double-blind cross-over study during 13 weeks in January--April 1973. The dose of SCG was 40 mg/day. Although a substantial number of results were obtained, no significant difference between the effects of SCG and placebo treatment, on variables measured, was detected. However, some patients experienced relief with, and preferred, the treatment with SCG. The results from this investigation are discussed in comparison with those from other studies including patients with vasomotor rhinitis.
Leukocyte migration inhibition by antigen from Candida albicans and Fusobacterium nucleatum was studied in man by means of the direct leukocyte migration agarose technique (LMAT). Antigens were prepared by prolonged ultrasonication and were added to the leukocyte cultures in the original as well as in the concentrated solutions. Significant inhibition of migration with a 10-fold concentrated solution of candida antigen was demonstrated in subjects showing apositive intracutaneous reaction to candida. With the fusobacterial antigen, inhibition of migration or stimulation of migration were demonstrated in subjects with slight peridontal inflammation. A 5-fold increase in the concentration of fusobacterial antigen resulted in increased stimulation of migration or inhibition of migration. Preincubation of the leukocytes with puromycin abolished both inhibition and stimulation of migration by candidal or fusobacterial antigen. The study confirms that it is important to study dose-related effects when applying the LMAT to test cell-mediated immunity. The study is in support of stimulation of migration being an immunologic reaction.
A leukocyte migration micromethod has been used to study the cellular immunity to penicillin (CIP) in newborns. No detectable CIP has been found in newborns who had not been treated with penicillin. Fifteen per cent of the newborns treated with pencillin were found to have a CIP as early as the 12th day of life.
In a pilot study, five adult patients with allergic rhinitis due to grass pollen underwent local intranasal hyposensitization with a chemically modified grass pollen extract--a so-called allergoid. Local hyposensitization during a pre-seasonal period resulted in an increased serum level of timothy-specific IgE antibodies in all patients, indicating that the allergoid had immunogenic properties. In four of the patients the clinical effect during the grass pollen season was judged satisfactory. Pre- and post-seasonal provocation test in these four patients also showed a reduction of the nasal sensitivity during this period. All patients tolerated the treatment well without any marked side effects. These promising preliminary results motivate further investigation into this form of therapy.
AllergyVolume 32, Issue 2 p. 118-138 SPORENKONZENTRATIONEN IN STÄDTEN DER EUROPÄISCHEN GEMEINSCHAFT (EG) II. Cladosporium- und Alternaria-Sporen N. BAGNI, N. BAGNI Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Search for more papers by this authorK. R. DAVIES, K. R. DAVIES Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Search for more papers by this authorM. MALLEA, M. MALLEA Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Search for more papers by this authorN. NOLARD, N. NOLARD Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Search for more papers by this authorF. T. SPIEKSMA, F. T. SPIEKSMA Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Search for more papers by this authorE. STIX, Corresponding Author E. STIX Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Anschrift der Verfasser: Dr. E. Stix, Auswertestelle Aerobiologie, Des Umweltbundesamtes, Türkenstrassse 38/II, D-8000 München 40, W. GermanySearch for more papers by this author N. BAGNI, N. BAGNI Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Search for more papers by this authorK. R. DAVIES, K. R. DAVIES Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Search for more papers by this authorM. MALLEA, M. MALLEA Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Search for more papers by this authorN. NOLARD, N. NOLARD Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Search for more papers by this authorF. T. SPIEKSMA, F. T. SPIEKSMA Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Search for more papers by this authorE. STIX, Corresponding Author E. STIX Aus Istituto ed Orto Botanico, Universitá di Bologna; The Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London; Clinique de Pneumo-Phtisiologie, Hopital Sainte-Marguerite, Marseille; Laboratoire de parasitologie, Faculté de Médecine et de Pharmacie, Unirersité Libre de Bruxelles, Bruxelles; Afdeling Allergologie, Academisch Ziekenhuis, Leiden; und Auswertestelle Aerobiologie des Umweltbundesamtes, München.Anschrift der Verfasser: Dr. E. Stix, Auswertestelle Aerobiologie, Des Umweltbundesamtes, Türkenstrassse 38/II, D-8000 München 40, W. GermanySearch for more papers by this author First published: April 1977 https://doi.org/10.1111/j.1398-9995.1977.tb02617.xCitations: 28AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Citing Literature Volume32, Issue2April 1977Pages 118-138 RelatedInformation
Both isobutylmethylxanthine and theophylline increased the level of cyclic AMP in rat mast cells. Theophylline reduced the allergic histamine release, whereas isobutylmethylxanthine caused a pronounced potentiation of the histamine release. This indicates that the hypothesis of an inverse relationship between the level of cyclic AMP in mast cells and histamine release is inadequate.
The purpose of the present investigation was to demonstrate in vitro cellular hypersensitivity to oral lichen planus lesions. Twenty oral lichen planus patients and 20 control persons matched by and sex were examined by the leukocyte migration agarose technique with tissue extracts from oral lichen planus lesions, normal oral mucosa and normal human kidney, the two latter extracts serving as control antigens. The female lichen planus patients showed statistically significant (P=0.004) migration inhibition with extracts from oral lichen planus lesions, which supports the hypothesis of cellular hypersensitivity to oral lichen planus lesions. However, the male lichen planus patients did not show any significant reaction. No significant reaction was found with the control antigens. The findings do not exclude the hypothesis of cellular hypersensitivity to oral lichen planus lesions. Furthermore, they do not support the hypothesis of oral lichen planus being an autoimmune disease.
AllergyVolume 32, Issue 5 p. 291-300 INFLUENCE OF THE SYNTHETIC PENTAPEPTIDE LAsp-Ser-Asp-Pro-Arg ON THE DIRECT AND INDIRECT MAST CELL DEGRANULATION INDUCED BY REAGINIC ANTIBODIES N. VARDINON, Corresponding Author N. VARDINON Department of Human Microbiology, Tel-Aviv University Sackler Medical School, Tel-Aviv, Israel.Nurith Vardinon Ph. D. Department of Human Microbiology Sackler School of Medicine Tel-Aviv University, Ramat-Aviv Tel-Aviv IsraelSearch for more papers by this authorZ. SPIRER, Z. SPIRER Department of Human Microbiology, Tel-Aviv University Sackler Medical School, Tel-Aviv, Israel.Search for more papers by this authorM. FRIDKIN, M. FRIDKIN Department of Human Microbiology, Tel-Aviv University Sackler Medical School, Tel-Aviv, Israel.Search for more papers by this authorJ. SCHWARTZ, J. SCHWARTZ Department of Human Microbiology, Tel-Aviv University Sackler Medical School, Tel-Aviv, Israel.Search for more papers by this authorS. BEN-EFRAIM, S. BEN-EFRAIM Department of Human Microbiology, Tel-Aviv University Sackler Medical School, Tel-Aviv, Israel.Search for more papers by this author N. VARDINON, Corresponding Author N. VARDINON Department of Human Microbiology, Tel-Aviv University Sackler Medical School, Tel-Aviv, Israel.Nurith Vardinon Ph. D. Department of Human Microbiology Sackler School of Medicine Tel-Aviv University, Ramat-Aviv Tel-Aviv IsraelSearch for more papers by this authorZ. SPIRER, Z. SPIRER Department of Human Microbiology, Tel-Aviv University Sackler Medical School, Tel-Aviv, Israel.Search for more papers by this authorM. FRIDKIN, M. FRIDKIN Department of Human Microbiology, Tel-Aviv University Sackler Medical School, Tel-Aviv, Israel.Search for more papers by this authorJ. SCHWARTZ, J. SCHWARTZ Department of Human Microbiology, Tel-Aviv University Sackler Medical School, Tel-Aviv, Israel.Search for more papers by this authorS. BEN-EFRAIM, S. BEN-EFRAIM Department of Human Microbiology, Tel-Aviv University Sackler Medical School, Tel-Aviv, Israel.Search for more papers by this author First published: October 1977 https://doi.org/10.1111/j.1398-9995.1977.tb02570.xCitations: 6 We thank Mrs. Nava Raz for her competent help. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL REFERENCES 1 Abramov, L. A., Yust, J., Fierstater, E. & Vardinon, N. (1976): Acute pulmonary distress due to hypersensitivity to erythromycin. Bull. Europ. Physiopath. resp. 12, 121. 2 Bahr-Lindstrom, H. & Bennich, H. (1974): Human immunoglobulin E. The primary structure of the third constant (CE3) domain of the epsilon chain. FEBS Letters 40, 57– 61. 3 Hamburger, R. N. (1975): Peptide inhibition of the Prausnitz Kustner reaction. Science 189, 387– 390. 4 Ishizaka, K., Tamioka, H. & Ishizaka, T. (1970): Mechanisms of passive sensitization. I. Presence of IgE and IgG molecules on human leukocytes. J. Immunol. 105, 1459– 1467. 5 Morton, H. J., Morgan, J. F. & Parker, R. C. (1963): In R. C. Parker, (ed): Methods of tissue culture, pp. 74– 75, Huebner, P. P. Inc., New York . 6 Perelmutter, L., Liakopoulou, A. & Phills, J. A. (1972): Studies on the interaction of human immunoglobulin E with rat mast cells. Immunol. Commun. 1, 431– 452. 7 Prausnitz, C. & Kuestner, H. (1921): Studien über die Überempfindlichkeit. Zbl. Bakt. Abt. I. (Orig.) 86, 160– 169. 8 Schwartz, J., Klopstock, A., Zickert-Duvdevani, P. & Honig, S. (1965): Detection of hypersensitivity by indirect mast cells degranulation. Int. Arch. Allergy 26, 333– 339. 9 Schwartz, J. & Vardinon, N. (1966): In vitro prevention of direct mast cell disruption by specific antibody. Int. Arch. Allergy 30, 67– 74. 10 Schwartz, J., Eyquem, A. & Vardinon, N. (1975): Mast cell sensitizing antibodies in Myastenia gravis, I. Immunol. Commun. 4, 243– 250. 11 Vardinon, N., Levanon, M. & Schwartz, J. (1967): The immunological value of the mast cell disruption inhibition test. Acta allergol. 22, 28– 38. Citing Literature Volume32, Issue5October 1977Pages 291-300 ReferencesRelatedInformation
Crushed, fresh grass did not produce positive skin reactions in 149 patients with grass pollen allergy, six of whom suffered from rhinitis and conjunctival discharge while cutting grass. An additional patient without an allergy to grass pollens but with clear-cut symptoms from newly cut grass showed a negative result in the scratch-chamber test and in the scrubbing test.
Supernatants from Con-A-stimulated human lymphocytes containing leukocyte migration inhibitory factor, skin reactive factor and procoagulant activities were tested for their effect on fibrinolysis and kinin generation. The supernatants of stimulated lymphocytes activated the fibrinolysis and generated kinin in human plasma. These results are discussed from the aspect of cell-mediated (type IV) immune inflammation.
An asthma data register which collects annually certain data from all known local asthmatic children has been presented. The data register is utilized to make a two-page print-out for every patient, and statistics of the whole material. These data make the continuous surveillance and guidance of the patients possible. The easy-to-use and accurate data material has contributed to rationalization of routine work in an asthma clinic. Team work has also improved because the uniform data material enables various workers to quickly pick out a specific problem from the whole material. The cost, $ 2 to 3 per patient per year, is low compared with the immediate benefit. Indirect benefit is to be expected because the system helps to investigate the problems of asthmatic children.
Transfer factor therapy was applied in three patients with severe atopic dermatitis and given at regular intervals for 1 1/2 years. Clinically, slight improvements were seen, attacks of impetigo ceased and admissions to hospital were not necessary. However, IgE concentrations in serum remained constantly high in all cases and the absolute number of T and B lymphocytes was continuously subnormal despite treatment. The in vitro cellular reactivity to PPD as assayed by a leucocyte migration test was not significantly altered in the patients, although a slight increase was found early on in the therapy. Finally, a serum factor inhibiting leucocyte migration and appearing simultaneously with attacks of impetigo disappeared during treatment. In conclusion, no convincing effect of transfer factor therapy was encountered in immune parameters and no major alterations were found in the status of the patients' atopic dermatitis.
The effect and tolerance of a new antihistamine, HC 20-511, in daily doses of 2 and 4 mg was compared with cyproheptadine (Periactin) 8 mg daily in 50 cases of chronic urticaria. HC 20-511 4 mg daily proved to be the most effective, rapid and long-lasting. HC 20-511 had less sedative effect than cyproheptadine, but both possessed the same appetite-stimulating effect. However, this was not noted with the smaller dosage of HC 20-511.
Dust from mattresses of different composition and age was analysed for mites, xerophilic fungi and allergenic activity. The mites of the genus Demodex were the most abundant (58.2 per cent). Also pyroglyphid mites occurred commonly (36.6 per cent). Pyroglyphid mites were present in small numbers (mean: 1 specimen/0.2 g of dust) in 12 out of the 17 older polyester-foam mattresses. The 11 cotton-horsechair mattresses and the newly used polyester-foam mattresses (three tested) were without them. The dust from the cotton-horsehair mattresses had a significantly higher allergenic activity than from those of polyester-foam. Xerophilic fungi were isolated in three out of 31 mattresses. The species isolated belonged to the genus Aspergillus and Eurotium. E. repens occurred most frequently. Disinfection of mattresses was suggested to have a negative influence on the occurrence of mites and fungi.
