Twenty-four strains mainly from lions, tigers and human wounds inflicted by these animals and strains from birds, elephants and a bite wound in a calf were investigated. As a consequence, the new species Pasteurella vulneris is proposed based on the phenotypic and genotypic similarity of strains, as well as differences to the other species of the genus. Monophyly was confirmed by partial 16S rRNA and rpoB gene sequence comparisons. The intra-species identity of P. vulneris 16S rRNA gene sequences was 99–100%, and the identity to strains of Pasteurella multocida was 99% or less. The identity to taxon 46-like of Bisgaard was 96–98%, to taxon 46 of Bisgaard 97–98%, and identities to Pasteurella stomatis , Pasteurella dagmatis , Pasteurella canis and Pasteurella oralis were within the range of 95–97%. The whole genomic sequences of ten strains obtained in a previously published study and analysed by the type strain genome server showed the highest digital DNA–DNA hybridization of 31% to the genome of the type strain of P. multocida and 59–93% between strains of P. vulneris . Average nucleotide identity was 95–98% between members of the new species with 87% to the type strain of P. multocida . Colonies of P. vulneris on bovine blood agar were regular, smooth, convex, opaque, greyish, non-haemolytic and circular after 24 h of incubation at 37 °C. Mucoid colonies are observed for some strains. Analysis of isolates by MALDI-TOF MS clustered them close together and separated them from other Pasteurella species, allowing discrimination and making this the method of choice for identification. The G+C content of the type strain HIM 1004-6 T isolated from a chipmunk (=Schmid351 T =CCUG 77952 T =DSM 118578 T ) is 40.78 mol%, calculated from the whole genome.
Salinomycin is a polyether ionophore discovered from a streptomycete strain designated 80614 (=DSM 41398=NBRC 107858), and the producer has been published as Streptomyces albus. In this study, the taxonomic position was investigated using a polyphasic approach. The 16S rRNA gene sequence of S. albus 80614 showed similarities of 99.8 and 98.8% to those of 'Streptomyces physcomitrii' LD120(T) and Streptomyces levis NBRC 15423(T), respectively, as the closest, but the similarity to S. albus NBRC 13014(T) was only 96.1%. S. albus 80614 was not close to S. albus NBRC 13014(T) and rather formed a clade with 'S. physcomitrii' LD120(T) in the phylogenetic tree based on 16S rRNA gene sequences. The digital DNA-DNA hybridization (dDDH) values were 67.8, 23.3 and 21.6% to 'S. physcomitrii' LD120(T), S. levis JCM 6924(T) and S. albus NBRC 13014T, respectively. The average nucleotide identity based on MUMmer (ANIm) was 96.5% between S. albus 80614 and 'S. physcomitrii' LD120(T). These dDDH and ANIm values were below the thresholds, 70 and 96.7% for bacterial and streptomycetal species delineation, respectively. The strain was differentiated from these three referenced species in major phenotypic characteristics such as spore morphology, NaCl tolerance and carbon source utilization. Therefore, Streptomyces salinomycinicus sp. nov. was proposed for the strain. This new species included S. albus ZD11, S. albus BK3-25, Streptomyces sp. SCSIO ZS0520, 'S. physcomitrii' NPDC020937 and 'S. physcomitrii' NPDC020938. Additionally, Streptomyces rubiginosus is reclassified as a later heterotypic synonym of Streptomyces pseudogriseolus; Streptomyces niveoruber as a later heterotypic synonym of Streptomyces griseoviridis; Streptomyces griseomycini as a later heterotypic synonym of Streptomyces griseostramineus; Streptomyces roseoviolaceus as a later heterotypic synonym of Streptomyces violaceus; Streptomyces violaceochromogenes as a later heterotypic synonym of Streptomyces collinus; and Streptomyces virens as a later heterotypic synonym of Streptomyces calvus.
Strain 7D2-24(T), an alkali-tolerant bacterium isolated from coastal sediments of Jiaozhou Bay, China, belongs to the genus Planococcus. The strain was Gramstain-positive, aerobic, motile and orange-pigmented. It was able to grow at 4-40 degrees C (optimum, 25-40 degrees C) and at pH 7.0-12.0 (optimum, pH 8.0-12.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 7D2-24(T) formed a lineage within the genus Planococcus with the closest phylogenetic neighbour Planococcus ruber CCTCC AB 207187(T) (98.39%). Digital DNA-DNA hybridization value and the average nt identity value of strain 7D2-24(T) with P. ruberCGMCC 1.19134(T) were 36.1 and 89.3%, respectively, falling below the species delineation thresholds of 70 and 95%. The genomic G+C content was 44.9 mol%. The predominant menaquinones were MK-7 (35.9%) and MK-8 (64.1%), and the major cellular fatty acids (>5% of the total) were anteiso-C-15:0 (39.2%), anteiso-C-17:0 (10.1%), iso-C-16:0 (8.7%) and iso-C-15:0 (5.6%). The major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphoglycolipid and three unknown lipids. Based on phenotypic, phylogenetic and genotypic data, a novel species, Planococcus alkaliphilus sp. nov., is proposed with the type strain 7D2-24(T) (=CGMCC 1.65191(T)=KCTC 43862(T)).
Twelve isolates representing two novel Acinetobacter species were isolated from various fresh meat and meat preparation samples collected from supermarkets in Le & oacute;n, Spain. These isolates were identified by matrix- assisted laser desorption/ionization time- of- flight mass spectrometry and GTDB- Tk software as members of the genus Acinetobacter. Phylogenomic analysis based on the core genome showed that these isolates formed two separate branches (1 including 11 isolates: ULE_I046T, ULE_I001, ULE_I024, ULE_I037, ULE_I053, ULE_I057, ULE_I064, ULE_I068, ULE_I075, ULE_I080 and ULE_I092; and the other only one, ULE_I010T) separated from all known Acinetobacter species. Average nucleotide identity and digital DNA-DNA hybridization values of all isolates with closely related type strains of species of the genus Acinetobacter supported their status as new species. The names Acinetobacter legionensis sp. nov. (comprising 11 isolates) and Acinetobacter psychrotolerans sp. nov. (comprising one strain) were proposed for the two novel taxa. Additional phylogenomic and phenotypic analyses showed that strain LMG 10606 (=RUH 2236), originally assigned to Acinetobacter guillouiae, belongs to A. legionensis sp. nov. Other two strains from the original description of A. guillouiae (LUH 5606 and LUH 6980) may also represent members of A. legionensis sp. nov., as indicated by a rpoB gene phylogenetic tree analysis. The phenotypic characteristics most useful for the differentiation of the novel species described in the present study from known Acinetobacter species were the assimilation of malonate and the inability to grow on histamine and phenylacetate of A. legionensis sp. nov., and the inability of A. psychrotolerans sp. nov. to grow over 30 degrees C. The type strain A. legionensis ULE_I046T (=CECT 31169T=CCP 535T) has a genome size of 4.58 Mb and a G+C content of 37.7 mol%. The type strain A. psychrotolerans ULE_I010T (=CECT 31168T=CCP 534T) has a genome size of 3.56 Mb, with a G+C content of 37.2 mol%.
This listing of names of prokaryotes published in a previous issue of the International Journal of Systematic and Evolutionary Microbiology (IJSEM) is provided as a service to microbiology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [1]. The names given herein are listed according to the Rules of priority (i.e. time and date of publication of the articles on the journal's platform and the order of valid publication of names in the original articles) [2].
