Respiratory Syncytial Virus (RSV) imposes a substantial health burden, particularly in infants and older adults, but comprehensive population-level data describing age-specific antibody responses alongside hospitalization patterns are scarce. To assess RSV seropositivity across demographic and geographic variables, to evaluate age-specific binding and neutralizing antibody responses, and to describe the age distribution of RSV-positive hospitalizations in an independent hospitalized cohort; any comparisons between the serosurvey and the hospitalized cohort are descriptive and hypothesis‑generating. A cross-sectional sero-survey of 1,396 sera samples was conducted to measure RSV binding antibodies using ELISA (values were defined as high-positive, borderline or low-positive). Of them, neutralizing antibodies were assessed in 243 age-stratified sera. An independent cohort of 1,232 RSV-positive hospitalized patients collected during the 2022–2025 winter seasons at a single center was used to evaluate age-specific hospitalization distributions; the two datasets were not linked at the individual level. Overall, the RSV high-seropositivity rate was 7.8
Fungal and parasitic infections exhibit considerable heterogeneity in their clinicopathological manifestations and geographic distributions. This study aimed to characterize the pathogen spectrum, clinicopathological features, anatomical distribution, and temporal trends of histopathologically confirmed fungal and parasitic infections in Zunyi, Guizhou Province, China. We retrospectively analyzed cases of histopathologically confirmed fungal and parasitic infections diagnosed at the Department of Pathology, Affiliated Hospital of Zunyi Medical University, between January 2014 and August 2026. Data on patient demographics, pathogen types, anatomical sites of infection, and histopathological findings were collected. Temporal trends in annual infection case counts and the proportions of fungal and parasitic infections were assessed using Spearman’s rank correlation analysis. A total of 1,817 cases were included, comprising 1,711 fungal infections (94.2
Tuberculosis-diabetes comorbidity is associated with altered host immunity and poorer clinical outcomes. Autophagy is relevant to intracellular mycobacterial control, but the transcriptional organisation of autophagy-related genes in tuberculosis-diabetes comorbidity remains incompletely defined. Public transcriptomic datasets related to tuberculosis-diabetes comorbidity were analysed, including the primary discovery set GSE114192 (TB-DM = 61, TB-only = 46, DM-only = 52, healthy controls = 36); drug-resistant-TB and lesion-tissue datasets were included only as complementary, cross-context resources rather than as a directly matched comorbidity or validation cohort. Autophagy-related genes were compiled from the Gene Ontology autophagy term and its descendants. A total of 310 autophagy-related genes were differentially expressed in the tuberculosis-diabetes versus healthy-control comparison (175 up-regulated, 135 down-regulated); 253 and 139 were altered in the TB-only and DM-only comparisons, and 78 genes were shared by all three. Protein interaction analysis of the prioritised genes identified a connected network (196 interactions among 60 genes) with MTOR, GABARAPL2, BCL2, RPTOR, ATG7 and ATG3 as the most central nodes, and pathway enrichment was dominated by canonical autophagy, mitophagy, lysosome and mTOR-related terms. A logistic model built on autophagy genes selected under nested cross-validation yielded an area under the receiver operating characteristic curve of 0.974 (95
Pneumococcal Disease (PD) is associated with significant health and economic burden in the US. The Advisory Committee on Immunization Practices (ACIP) recommends pneumococcal vaccination for adults with immunocompromised conditions and chronic medical conditions who are at increased risk of PD. This study examines the distribution of PD risk conditions among US adults by single age cohorts, based on the ACIP classification of conditions. A retrospective observational study was conducted among individuals enrolled in US IBM@ MarketScan Commercial Claims and Encounters (CCAE) in 2022. The risk of PD (low-risk (LR), moderate-risk (MR), and high-risk (HR) of PD) among adults aged ≥ 18 years was classified using International Classification of Diseases, Clinical Modification version 10 (ICD-10-CM) and Current Procedural Terminology version 4 codes, based on a 5-year lookback period among individuals with continuous enrollment. Risk distributions were summarized by single-year age cohort, and the clinical conditions comprising the MR and HR categories were described. Supplementary analyses summarized risk distributions using broader age categories, and sensitivity analyses assessed the impact of using a shorter 2-year lookback period and removing the continuous enrollment requirement. The study population included more females (52.2
Tropheryma whipplei (TW), the pathogen of Whipple’s disease, has recently been identified as potentially pathogenic in respiratory infections through metagenomic next-generation sequencing (mNGS) technology. However, its clinical significance in lung infections is still unclear due to its frequent co-detection with other pathogens and the lack of diagnostic standards. We retrospectively analyzed 74 patients with TW-positive bronchoalveolar lavage fluid (BALF) detected by mNGS from January 2018 to December 2022. Over the study period, a total of 1,543 BALF samples were subjected to mNGS analysis, resulting in a detection rate of 4.79
