Aiming at the problems of low uniformity and utilization rate in the traditional deep application method of orchard manure, a soil–fertilizer collision mixing and mulching device was designed. The ditching mechanism, the structure of the soil divider, and the fertilizer delivery auger were analyzed and designed. Furthermore, with the rotational speed of the cutter and auger and the deflection angle of the soil divider as factors, as well as the uniformity of soil–fertilizer mixing and mulching as evaluation indexes, the discrete element simulation tests were conducted. The simulation results showed that when the turret speed, the stirrer speed and the soil separator deflection angle were 140 r/min, 146 r/min and 22°, the mixing uniformity and mulching uniformity were the highest, which was 88.35% and 96.86%, respectively. Based on the optimal parameters, the field test was conducted, and the soil–fertilizer mixing uniformity was 87.02%, with a relative error of 1.33% compared with the simulation test results. The relative error of 94.37% of mulch uniformity is 2.49%, which indicates that the simulation optimization results are reliable and the mixing performance of the device is good and can meet the requirements of soil–fertilizer mixing operation. The results of this study can provide an important reference for the design of the soil and fertilizer mixing machine.
Objective To investigate the effect of IGF2BP3(insulin-like growth factor 2-mRNA binding protein 3)overexpression on hepatoma cell proliferation and its possible molecular mechanism,and to provide experimental basis and theoretical basis for clinical treatment of hepatoma.Methods mRNA and protein expression levels of IGF2BP3,AKT,p-AKT,S6 and p-S6 were detected in HuH-7 and MHCC97H HCC cells transfected with IGF2BP3 overexpression plasmid.CCK-8 and EdU assay were used to detect cell proliferation.The effect of IGF2BP3 on tumor growth was detected by subcutaneous tumorigenization test,and the expressions of IGF2BP3 and Ki67 in overexpressed IGF2BP3 group and control group were detected by IHC.Results After transfection of IGF2BP3 overexpression plasmid,the mRNA and protein expression levels of IGF2BP3 were significantly increased with statistical significance(P<0.001),and overexpression of IGF2BP3 promoted the proliferation of liver cancer cells(P<0.01).Overexpression of IGF2BP3 promoted the growth of liver cancer,and immunohistochemistry showed that the expressions of IGF2BP3 and Ki67 were up-regulated.Western Blot showed that the expressions of p-AKT and p-S6 were up-regulated.The proliferation ability of hepatocellular carcinoma cells was significantly decreased after AKT inhibition(P<0.01).Conclusion Overexpression of IGF2BP3 may promote the prolifera-tion of liver cancer by activating PI3K/AKT/mTOR signaling pathway.
Objective Studies had found that Safflower yellow(SY)has neuroprotective effects on various AD models,but the effective target and potential molecular mechanism of safflower yellow in treating AD are still unclear.Therefore,by establishing Aβ 1-42 induced AD model rats were treated with SY to explore the possible mechanism of SY anti-AD.Methods Morris water maze experiment was used to observe the improvement of SY on learning and memory loss in AD model rats;Golgi staining was used to determine the effect of SY on the morphology of dendritic spines in the hippocampus of AD model rats;Quantitative proteome method was used to identify and quantify the proteins in the hippocampus of rats in each group and analyze the differentially expressed proteins through bioinformat-ics.Results The results showed that SY could significantly improve the learning and memory ability of AD model rats and improve the morphological damage of dendritic spines.The results of proteomic analysis showed that Mvd,Map6d1 and Rpl34 were three important differentially expressed proteins in the two comparative groups,which were closely related to the pathogenesis of AD,and the results of Western Blot validation were consistent with the results of proteomic analysis.Conclusion(1)"Ribosomes"and"complement and co-agulation cascades"may be involved in Aβ1-42-induced nerve damage;(2)Rpl34,Mvd and Map6d1 may be the key molecular tar-gets of SY anti-AD;(3)SY may play a neuroprotective role by influencing the"ECM-receptor interaction".
Function space with variable exponents is widely concerned because of its important applications in equations with non-standard growth conditions.In this paper,by the use of space embedding,log-Hölder continuity of variable exponent and some related properties of variable weight function,the authors obtain the duality of the weighted grand Herz spaces with variable exponents.Fur-thermore,the boundedness of some sublinear operators in these spaces is also considered.
Gibberellin and forchlorfenuron,as efficient plant growth regulators,are widely used in fruit enlargement and seedless pro-duction.In order to explore the inhibition of cell growth and mutagenic toxicity of gibberellic acid and forchlorfenuron after contact with cells,and to evaluate the damage of gibberellic acid and forchlorfenuron,the cytotoxicity of gibberellic acid and forchlorfenuron at the cellular level was preliminarily explored by using human normal liver cells(WRL68).The mutagenicity of gibberellic acid and for-chlorfenuron was evaluated by TK gene mutation test of mouse lymphoma cells(L5178Y)and Ames test of Salmonella typhimurium.The research results indicated that the IC50 of GA and forchlorfenuron on WRL68 cells were 1.377 mg·mL-1 and 57.72 μg·mL-1,re-spectively.Meanwhile,No mutagenic toxicity was observed that at 4 mg·mL-1 gibberellin and 100 μg·mL-1 forchlorfenuron of dosage.Gibberellin and forchlorfenuron inhibited the proliferation of WRL68 cells in varying degrees,but there was no mutagenic toxicity.
