Aiming at the problems of low uniformity and utilization rate in the traditional deep application method of orchard manure, a soil–fertilizer collision mixing and mulching device was designed. The ditching mechanism, the structure of the soil divider, and the fertilizer delivery auger were analyzed and designed. Furthermore, with the rotational speed of the cutter and auger and the deflection angle of the soil divider as factors, as well as the uniformity of soil–fertilizer mixing and mulching as evaluation indexes, the discrete element simulation tests were conducted. The simulation results showed that when the turret speed, the stirrer speed and the soil separator deflection angle were 140 r/min, 146 r/min and 22°, the mixing uniformity and mulching uniformity were the highest, which was 88.35% and 96.86%, respectively. Based on the optimal parameters, the field test was conducted, and the soil–fertilizer mixing uniformity was 87.02%, with a relative error of 1.33% compared with the simulation test results. The relative error of 94.37% of mulch uniformity is 2.49%, which indicates that the simulation optimization results are reliable and the mixing performance of the device is good and can meet the requirements of soil–fertilizer mixing operation. The results of this study can provide an important reference for the design of the soil and fertilizer mixing machine.
Objective To investigate the effect of IGF2BP3(insulin-like growth factor 2-mRNA binding protein 3)overexpression on hepatoma cell proliferation and its possible molecular mechanism,and to provide experimental basis and theoretical basis for clinical treatment of hepatoma.Methods mRNA and protein expression levels of IGF2BP3,AKT,p-AKT,S6 and p-S6 were detected in HuH-7 and MHCC97H HCC cells transfected with IGF2BP3 overexpression plasmid.CCK-8 and EdU assay were used to detect cell proliferation.The effect of IGF2BP3 on tumor growth was detected by subcutaneous tumorigenization test,and the expressions of IGF2BP3 and Ki67 in overexpressed IGF2BP3 group and control group were detected by IHC.Results After transfection of IGF2BP3 overexpression plasmid,the mRNA and protein expression levels of IGF2BP3 were significantly increased with statistical significance(P<0.001),and overexpression of IGF2BP3 promoted the proliferation of liver cancer cells(P<0.01).Overexpression of IGF2BP3 promoted the growth of liver cancer,and immunohistochemistry showed that the expressions of IGF2BP3 and Ki67 were up-regulated.Western Blot showed that the expressions of p-AKT and p-S6 were up-regulated.The proliferation ability of hepatocellular carcinoma cells was significantly decreased after AKT inhibition(P<0.01).Conclusion Overexpression of IGF2BP3 may promote the prolifera-tion of liver cancer by activating PI3K/AKT/mTOR signaling pathway.
Objective Studies had found that Safflower yellow(SY)has neuroprotective effects on various AD models,but the effective target and potential molecular mechanism of safflower yellow in treating AD are still unclear.Therefore,by establishing Aβ 1-42 induced AD model rats were treated with SY to explore the possible mechanism of SY anti-AD.Methods Morris water maze experiment was used to observe the improvement of SY on learning and memory loss in AD model rats;Golgi staining was used to determine the effect of SY on the morphology of dendritic spines in the hippocampus of AD model rats;Quantitative proteome method was used to identify and quantify the proteins in the hippocampus of rats in each group and analyze the differentially expressed proteins through bioinformat-ics.Results The results showed that SY could significantly improve the learning and memory ability of AD model rats and improve the morphological damage of dendritic spines.The results of proteomic analysis showed that Mvd,Map6d1 and Rpl34 were three important differentially expressed proteins in the two comparative groups,which were closely related to the pathogenesis of AD,and the results of Western Blot validation were consistent with the results of proteomic analysis.Conclusion(1)"Ribosomes"and"complement and co-agulation cascades"may be involved in Aβ1-42-induced nerve damage;(2)Rpl34,Mvd and Map6d1 may be the key molecular tar-gets of SY anti-AD;(3)SY may play a neuroprotective role by influencing the"ECM-receptor interaction".
Function space with variable exponents is widely concerned because of its important applications in equations with non-standard growth conditions.In this paper,by the use of space embedding,log-Hölder continuity of variable exponent and some related properties of variable weight function,the authors obtain the duality of the weighted grand Herz spaces with variable exponents.Fur-thermore,the boundedness of some sublinear operators in these spaces is also considered.
Gibberellin and forchlorfenuron,as efficient plant growth regulators,are widely used in fruit enlargement and seedless pro-duction.In order to explore the inhibition of cell growth and mutagenic toxicity of gibberellic acid and forchlorfenuron after contact with cells,and to evaluate the damage of gibberellic acid and forchlorfenuron,the cytotoxicity of gibberellic acid and forchlorfenuron at the cellular level was preliminarily explored by using human normal liver cells(WRL68).The mutagenicity of gibberellic acid and for-chlorfenuron was evaluated by TK gene mutation test of mouse lymphoma cells(L5178Y)and Ames test of Salmonella typhimurium.The research results indicated that the IC50 of GA and forchlorfenuron on WRL68 cells were 1.377 mg·mL-1 and 57.72 μg·mL-1,re-spectively.Meanwhile,No mutagenic toxicity was observed that at 4 mg·mL-1 gibberellin and 100 μg·mL-1 forchlorfenuron of dosage.Gibberellin and forchlorfenuron inhibited the proliferation of WRL68 cells in varying degrees,but there was no mutagenic toxicity.
Gauss-Weierstrass operator is a very important approximation tool in approximation theory,and it is also the main content of harmonic analysis.In practical applications,the Gauss-Weierstrass operator can be used to realize the low-pass filtering of the image,so as to achieve the effect of image smoothing.Scholars at home and abroad mainly study the discussion of Gauss-Weierstrass operator in Lpspace and Besov space.The discussion of the linear combination of Gauss-Weierstrass operators in Orlicz space is a difficult prob-lem,and there are few research results.In this paper,we mainly study the linear combination of Gauss-Weierstrass operators with Ja-cobi weights.By using Hölder inequality,Jensen inequality,Hardy-Littlewood maximal function and K-functional,we derive the ap-proximation theorem of Jacobi weight function of the linear combination of Gauss-Weierstrass operators in Orlicz space.
Objective To investigate the effect of NFATc3 gene knockout on the transcriptome of liver cancer cells,and to preliminarily screen the target genes regulated by NFATc3,to provide a basis for identifying NFATc3 target genes and finding new targets for anti-liver cancer therapy.Methods The SMMC7721 cells with NFATc3 knocked out were used to perform RNA sequencing(RNA-Seq)with or without Sendai virus(SeV)infection,and different expression genes were subjected to GO and KEGG enrichment analysis.PPI protein network interaction analysis was performed on immune-related genes in the intersection of different expression genes from the two sequencing data.qRT-PCR experiments were used to validate the effect of NFATc3 knockdown or overexpression on the expres-sion levels of S100A8 and S100A9 in liver cancer cells.The mRNA expression levels and prognosis of S100A8 and S100A9 in liver cancer were analyzed using database data.Results RNA-seq analysis revealed that the different expression genes in liver cancer cells with NFATc3 knockout were mainly enriched in pathways related to development,metabolism,extracellular matrix,and angiogenesis.PPI protein network analysis identified S100A8 and S100A9 as potentially important target genes of NFATc3.qRT-PCR experiments showed that knocking out or knocking down NFATc3 could upregulate the expression of S100A8 and S100A9 while expressing exoge-nous NFATc3 could downregulate the expression of S100A8 and S100A9.Database analysis showed that the expression levels of S100A8 and S100A9 in liver cancer tissues were significantly higher than those in adjacent tissues,and the high expression of S100A8 and S100A9 in liver cancer patients was associated with shorter survival time and poorer prognosis.Conclusion The knockout of the NFATc3 gene in liver cancer cells leads to functional enrichment of host genes primarily involved in pathways related to development,metabolism,extracellular matrix,and angiogenesis.NFATc3 can inhibit the transcriptional expression of S100A8 and S100A9 genes,and S100A8 and S100A9 may be potential target genes for NFATc3 regulation in liver cancer.
As a rigid and flexible composite geomembrane-cushion impervious structure,the interface friction characteristics of the composite geomembrane-cushion impervious structure are very important to the safe operation of engineering.To provide a scientific and reasonable basis for the application of composite geomembrane impermeability in the engineering of large flow diversion channel,explore the interface friction characteristics and interface strength characteristics of composite geomembrae-bedding,the change of water content of bedding material behind the membrane was mainly considered,the indoor direct shear test was conducted to study the friction shear characteristics of composite geomembrae-bedding material with different water content(0%,3%,6%,8.5%),and the interface strength characteristics under different water content conditions were discussed.The results show that the interfacial shear properties of composite geomembranes and bedding materials with different water content go through elastic,elastoplastic and residual strength stages,and have obvious peak strength and residual strength characteristics.The peak strength is reached when the shear displacement is 9～11 mm.With the increase of shear displacement,the shear stress at the interface decreases first and then tends to be stable,showing a stable residual strength,which is about 80%～90%of the peak strength.The peak shear strength and residual shear strength at the interface showed a nonlinear decreasing trend with the increase of the moisture content of the bedding material,indicating that the moisture content of the bedding material behind the film had a"lubricating effect"on the interface strength.
Micromixing performance in microchannel mixer is one of the key factors for fast reaction systems,such as preparation of polymeric nanocomposites.Its micromixing efficiency is a key parameter indicator for the application and optimization of microchannel mixers,which is one of parameters has received significant attention.This work,the micro-structured multichannel mixer was construc-ted,and fabricated by a stereolithography 3D printing molding technology,which has the advantages of enhanced mixing and ease of manufacture.This study employed the iodide-iodate reaction system and computational fluid dynamics(CFD)method to analyze the micromixing efficiency in microstructured multi-channel mixer,and investigated the effect of Reynolds number(Re)in the multi-chan-nel mixing chamber on the micromixing efficiency.The separation index(XS)was used to evaluate the micromixing efficiency.The re-sults showed that XS decreases with increasing Reynolds number,the center of the chamber has low turbulence intensity in low Re,the region of highest turbulent kinetic energy moves towards the center of the chamber and effective mixing is achieved when Re reaches 4963.Excellent correspondence is found between the simulation and the experimental results.This study indicated that the mixer has advantages in terms of improved micromixing efficiency and fabrication methods,it is therefore expected to have applications in fast re-action systems and enhanced mixing processes.
In order to investigate the seismic performance and failure mechanism of the desert sand concrete frame structure,9 two-story planar frame model based on ABAQUS finite element software was established to study the effects of material type,concrete floor slab,stirrup ratio and axial compression ratio on the seismic performance of desert sand concrete frames.The results showed that the bearing capacity,initial stiffness and post-yield bearing capacity margin of desert sand concrete frame were similar to those of ordinary sand concrete frame;compared with desert sand concrete frame without slab,the energy dissipation increased by about 20%and the bearing capacity increased by about 6%;when the axial compressive ratio increases from 0.2 to 0.6,the deformation capacity of the specimen decreased by nearly 15%;when the stirrup ratio increased by 1.23%,the yield load of the structure increased by about 1.5%,and the ultimate load growth decreased by about 2%.
Objective To investigate whether autophagy inducer can reduce ferroptosis of mouse pancreatic β-cells induced by high-glu-cose and high-lipid through activation of autophagy.Methods The pancreatic β-cell injury model was established by treating high glu-cose(25 mmol·L-1)and sodium palmitate(200 μmol·L-1)(GP)in MIN6 cell.And on this basis,treated with rapamycin(RAPA)and erastin(Era).And the cell viability was detected by CCK8 assay.Furthermore,ferrous ion,the concentration of reactive oxygen species(ROS),malondialdehyde(MDA),glutathione(GSH)and the activity of superoxide dismutase(SOD)were detected by com-mercial kit.Glutathione Peroxidase 4(GPX4)and acyl-CoA synthetase long-chain family member 4(ACSL4),ferritin(Ferritin,FE)and Microtubule-associated protein 1 light chain 3Ⅱ(LC3Ⅱ)and P62 were detected by Western blot.Results GP and Era inhibited MIN6 cell viability,GSH content,SOD activity,increased ROS level,MDA and ferrous ion content,decreased GPX4 protein expres-sion level and increased ACSL4,LC3II,P62,FE protein expression level(P<0.05).RAPA intervention increased cell viability,de-creased ferrous ions,MDA and ROS levels,increased GSH,SOD content,increased GPX4,LC3Ⅱ protein expression,and decreased ACSL4,P62,and FE protein expression(P<0.05).Conclusion Ferroptosis is involved in GP-induced MIN6 cell damage,and the mechanism may be related to autophagic flux blockage.Rapamycin may improve β-cell ferroptosis.
A series of novel β-carbolines possessing different substituents at R6 and N9 positions were synthesized from the L-trypto-phan.All newly synthesized compounds were characterized by nuclear magnetic resonance(NMR)and High resolution mass spectra(HRMS),and antitumor activities against A549(lung carcinoma),BGC-823(gastric carcinoma),CT-26(murine colon carcino-ma),Bel-7402(liver carcinoma)and MCF-7(breast carcinoma)cell lines in vitro were investigated by methyl thiazolyl tetrazolium(MTT)assay.The results demonstrated that compounds 6l-6n and 6t possessing electron-withdrawing substituents(e.g.,3,4,5-trif-luorophenyl,6-cyanophenyl)at the R6-position,were found to be the most potent compounds(IC50 value lower than 10 μmol·L-1 against the investigated 2 cancer cell lines).Furthermore,In order to understand the combination,molecular docking was performed to investigate their binding mode and affinities towards VEGFR-2,PDGFR-α,and KIT kinase.
The study on grassland coverage and its spatial heterogeneity in Manas River Basin is helpful to timely grasp the current situ-ation of grassland in arid areas,and is of great significance for grassland protection,ecological restoration and construction.Based on the data of 9 types of natural grassland and MODIS NDVI data of from 2001 to 2020 in the basin,this study uses the pixel dichotomy model and trend analysis method to analyze the coverage and dynamic change trend of different grassland types.And based on the data of climate,topography and soil,the spatial heterogeneity of grassland coverage and natural environment factors is analyzed by geograph-ical weighted regression model.The results show that:(1)In the past 20 years,the grassland coverage in Manas River Basin has shown a significant increasing trend,with an average annual value of 28.78%and a growth rate of 0.003 2/20 a.The high value is concentrated in the southern mountain area of about 43.5° N,and the low value is concentrated in the northern desert area;Among the grassland types in the basin,the mountain meadow has the highest coverage with a value of 67.13%,while the temperate desert has the lowest coverage with a value of 23.33%.(2)The area of increasing and decreasing grassland coverage in Manas River Basin account for 75.39%and 24.31%of the total grassland area,respectively.Among different grassland types,the temperate desert in the western basin has the widest improvement area,and the degradation of mountain meadows is the most severe.(3)The annual average precipi-tation and soil organic carbon content mainly have a positive impact on grassland coverage,while the annual average temperature,alti-tude variation,soil acidity and alkalinity,and soil sand content mainly have a negative impact.The interaction between various factors and grassland coverage shows significant spatial differences.Precipitation and altitude variation mainly affect the grassland coverage in the northern basin.Temperature and precipitation affect grassland coverage in the central of basin.Temperature,precipitation,and soil sand content affect grassland coverage in the southern of basin.
Under the background of global warming and ecological protection,the aeolian sand activity in Gurbantunggut Desert has weakened and many dunes have been eroded.In this paper,we selected thirteen fixed barchan dunes in different developmental stages in the western Gurbantunggut Desert,and collected the surface sand samples from different parts of the dunes for grain size and quartz sand surface microstructure analysis.The results show that:(1)the surface sand of the dunes is more than 50%fine sand,the average particle size ranges from 2.03 to 2.88 Φ,with good sorting,near symmetry skewness and medium kurtosis.The grain size parameters of the windward slope were irregular,and the average grain size at the top of the dune was the thickest.With the maturity and increase of the scale of the barchan dunes,the fluctuation range of the surface sand components increases,the average particle size becomes coarser,the sorting becomes worse.(2)The roundness of gravel particles in sand dunes was generally poor,and there were a variety of structural characteristics of the native environment,which reflected the complex and diverse material sources.Although the sand was transported and accumulated by wind,the maturity of the sand was still low because the study area is in the downstream of the river,the source is updated frequently.Moreover,the desert vegetation coverage is relatively high,the dune mobility is poor,and the trans-portation distance are limited.Therefore,the aeolian sand preserves more traces of the original environment.(3)The characteristics of grain size and quartz sand microstructure on the surface of dunes in the study area has the uniqueness of erosive dunes and the particu-larity under the influence of vegetation cover.While grain size parameters showed irregular changes,the vegetation greatly reduced the difference of grain size distribution characteristics of dunes in different developmental stages.It also leads to poor roundness of quartz sand and rich traces of original environment.
针对全混合日粮搅拌机工作过程中存在的工作部件易磨损等问题,为全混合日粮搅拌机设计与优化提供参考依据,本文以含水率为试验因素,利用英斯特朗 8801 型万能试验机对不同含水率下不同品种甘草茎秆开展了压缩、蠕变与应力松弛特性试验研究.试验结果表明:当含水率为 50%时,光果甘草茎秆在弹性阶段的最大抗压强度为 10 MPa;随着含水率的增加,甘草茎秆的蠕变曲线逐渐向上移动,当含水率为 50%时光果甘草茎秆的蠕变曲线明显高于其他 2 个品种,而在其他含水率时 3 个品种的蠕变曲线较为相似,说明 Burgers四元件模型可以很好的反映甘草茎秆的蠕变特性;随着含水率的增加,甘草茎秆应力松弛曲线逐渐向下移动,胀果甘草茎秆的应力松弛曲线在 3 个含水率下均高于其他 2 个品种,Maxwell五元件模型可以很好的反映甘草茎秆的应力松弛特性.
目的 观察防己黄芪汤对气虚水负荷模型大鼠的利尿作用及肾脏水通道蛋白(Aquaporin,AQP)1～4 表达的影响.方法 采用"游泳疲劳+饮食失节"复合因素诱导制备气虚大鼠模型,生理盐水造成水负荷状态,设防己黄芪汤低、中、高剂量组,另设空白对照组,每组 10 只.观察各组大鼠一般状态及体重变化,用显微镜观察各组大鼠的肾脏病理变化,Real-Time PCR法和 Western Blot法检测各组大鼠肾脏 AQP1～4 的 mRNA和蛋白表达.结果 给药后,与模型组比较,防己黄芪汤中、高剂量组一般状态良好,体重明显增长(P<0.05).肾脏病理检测结果显示,模型组有肾小管扩张化,其余各组未见异常变化.Real-Time PCR和 Western Blot结果显示,与空白对照组比较,模型组大鼠肾脏组织 AQP 1～4 mRNA和蛋白表达明显升高(P<0.05);与模型组比较,防己黄芪汤中、高剂量组大鼠肾组织AQP 1～4 mRNA和蛋白表达明显降低(P<0.05).结论 防己黄芪汤对气虚水负荷模型大鼠有明显的利尿作用,这可能与下调肾 AQP 1～4 的 mRNA和蛋白表达有关.
本文的目的是在已知随机变量某些矩信息的条件下,给出随机变量函数的方差和相应协方差的半参数界.本文应用对偶原理,给出了美式蝶式期权和欧式蝶式期权的方差的上界估计.运用等价公式,测度变换,找到控制函数,分别给出了单峰分布下欧式看涨期权与美式蝶式期权的协方差的下界估计以及欧式看涨期权与欧式蝶式期权的协方差的上界估计.
针对决策信息为区间值 Picture模糊数的多属性群决策问题,在充分考虑属性间相互关联的情况下,将区间值Picture模糊数与广义 Heronian均值函数和 Heronian平均算子结合,定义了区间值 Picture模糊参数广义加权 Hero-nian平均算子,并研究了其性质.该信息集结算子能够反映信息的完整性、属性相关性和决策者的风险偏好.然后,提出了一种基于区间值 Picture模糊参数广义加权 Heronian平均算子的多属性群决策模型.最后,运用本文提出的群决策模型对信息系统进行了最优选择,并与其它模型进行比较,说明所提模型的有效性和优越性.
目的 筛选、鉴定绵羊卵巢差异 MicroRNA(miRNA)并研究其调控机制,为绵羊非繁殖季节发情的研究奠定基础.方法 使用 Solexa测序技术进行筛选和分析,对发情和乏情绵羊卵巢,差异 miRNA 的数量和特征进行鉴定,通过实时荧光定量 PCR进行检测.还进行了对 miR-200c靶基因检测、GO 注释和 KEGG 信号通路富集等的研究工作.并通过 TargetScan技术、RNAhybrid技术等,成功找到了 MAPK8-3′端非翻译区(3′UTR)与 miR-200c的潜在互补结合位点,PCR扩增 MAPK8(丝裂原活化蛋白激酶 8)基因的 3′UTR 序列,并成功实现了在 psiCHECK2 的克隆,成功建立了 MAPK8 野生型/突变型重组的双荧光素酶报告质粒.接着把野生型 psiheck2-MAPK8-3′UTR/突变型psiheck2-MAPK8-mut3′UTR的质粒,与 miR-200c mimics/mimics-NC,转染至 HEK 293T细胞中,从而通过双荧光素酶的系统,测定双荧光素酶的生物活性.结果 建立了 OAN(乏情)和 OEN(发情)非繁殖季节绵羊卵巢文库.鉴定出 113 个差异miRNA,9 个为已知miRNA,104 个为未知miRNA.在已知miRNA中有 6 个miRNA显著(P<0.05)上调、3 个下调,其中上调倍数最高的为 miR-200c.在 104 个未知的 miRNA中,有 52 个 miRNA显著(P<0.05)上调,52 个显著(P<0.05)下调.获得候选新 miRNA的长度分布主要集中在 21～23 nt 之间.荧光定量证实,miRNA 表达的趋势与测序结果一致.用软件预测到 miR-200c的 27 个靶基因.GO 注释发现 miR-200c 在卵巢组织中介导细胞增殖、迁移、凋亡等过程.KEGG分析表明,miR-200c的靶基因涉及发情相关途径(MAPK信号途径、胰岛素信号通路和 GnRH信号途径)以及与卵泡/黄体发育相关的途径,MAPK通路是富集基因最多的通路,共有 25 个基因被富集.干扰 miR-200cmimics,会引起 MAPK8 基因的 Wt型质粒的荧光表达明显减少(P<0.05),而 Mut型则没有明显改变.结论 筛选出非繁殖季节发情差异显著(P<0.05)的 miRNA有 6 个.试验研究初步证实了 miR-200c与MAPK8 的靶向关系,开展了 miRNA家族调节绵羊非繁殖季节发情的试验,为深入研究其调控绵羊繁殖及卵泡发育机制提供了试验基础.
为了研究沙漠砂再生混凝土(DSRC)在冻融环境下的耐久性能,探明沙漠砂(DS)和再生粗骨料(RCA)替代率对其冻融后力学性能的影响规律,并为 DSRC在新疆等寒冷地区构件和结构设计提供数据支撑,对 DSRC进行冻融后轴心抗压强度试验,分析冻融循环作用下 DSRC质量损失率、动弹性模量损失率和应力-应变曲线的变化规律.结果表明:在冻融循环次数相同时,DSRC动弹性模量损失率、极限应变和弹性模量均小于普通混凝土,峰值应力大于普通混凝土;随着冻融循环次数增加,DSRC质量损失较小,动弹性模量损失率、峰值应变和极限应变均增加,峰值应力和弹性模量均减小;DS和 RCA替代率分别为 40%时,DSRC质量损失率和动弹性模量损失率分别为 0.25%和 27.50%,其抗冻性能最优;采用普通混凝土本构模型对 DSRC 应力-应变曲线进行拟合,相关控制参数表明曲线预测能力良好,可对冻融后 DSRC的力学性能进行分析.
