Retraction Notice:The authors have requested retraction following the identification of errors in the data.Reference:Xian Wang, Shan Wu Feng, Fuzhou Wang, Shiqin Xu, Modeled Behavioral Evaluation of the Neuropathic Pain with Social Defect in Rats:A Preliminary Methodology Evaluation. Med Sci Monit Basic Res. 2014; 20: 164-169. DOI: 10.12659/MSMBR.892615.
BACKGROUND Acromioclavicular (AC) joint dislocation is a common shoulder injury, with a range of suture button techniques available for treatment. This study aimed to evaluate the clinical outcomes of a novel suture button technique - the FengChao button technique. MATERIAL AND METHODS Between December 2019 and December 2021, 25 patients with AC joint dislocation were treated using the arthroscopic triple FengChao button technique. Clinical outcomes measured included UCLA and CMS scores, operative time, and the incidence of complications. Radiological assessment focused on the coracoclavicular (CC) distance. RESULTS The follow-up period ranged from 6 to 19 months, with an average of 13.3 months. One patient required an open revision due to postoperative trauma. The mean operative time was 52.34±13.76 minutes. The UCLA score improved significantly from a preoperative average of 19.32±2.45 to 31.67±2.38 at the final follow-up (P<0.05). Similarly, the CMS score increased from 75.23±2.34 preoperatively to 92.73±2.96 at the final follow-up (P<0.05). The CC distance narrowed from a preoperative measurement of 21.34±1.32 mm to 9.23±1.13 mm 2 to 4 days postoperatively (P<0.05), and further to 11.93±1.03 mm at the final follow-up (P<0.05). CONCLUSIONS The arthroscopic triple FengChao button technique for AC joint dislocation offers satisfactory short-term functional outcomes.
BACKGROUND This study aims to explore the therapeutic mechanisms of Jinshuiqing (JSQ) in IgA nephropathy (IgAN) using transcriptomic analysis and animal experimentation. MATERIAL AND METHODS Six-week-old male C57BL/6 mice (20±2 g) were divided into 2 groups: IgAN model and JSQ-treated. The IgAN model was induced in SIRT3 knockout mice with acidified BSA, CCl4, castor oil, and LPS injections. The IgAN group received saline, while the JSQ group was treated with JSQ (4 g/kg/day) for 11 weeks. Following euthanasia, kidney tissues were collected for transcriptomic analysis. RNA extraction and sequencing were performed, followed by differential gene expression and pathway enrichment analysis to assess JSQ's effects. RESULTS PCA and correlation analysis showed distinct differences between the JSQ-treated and control groups. Differential gene expression identified 118 genes with significant changes, indicating that JSQ induces transcriptional alterations. Gene ontology (GO) and KEGG enrichment analyses revealed that JSQ affects pathways related to inflammation, oxidative stress, and metabolism, suggesting its potential to improve kidney function and regulate immune responses in IgAN. CONCLUSIONS JSQ treatment modulates gene expression in IgAN mice, influencing pathways linked to inflammation, gut microbiota, and kidney function. These findings provide insight into how JSQ alleviates inflammation and promotes renal health, offering genetic evidence for its therapeutic efficacy in IgAN.
BACKGROUND Childhood asthma, especially in the youngest children, is difficult to diagnose and is often based on the patient's symptoms. This study compared metabolite concentrations in serum samples between 13 children with asthma and IgE-dependent allergy and 10 without asthma and IgE-dependent allergy, aged between 6 months and 5 years. MATERIAL AND METHODS The study included a total of 23 children. The targeted metabolomic analysis was performed using an AbsoluteIDQ p180 kit and analyzed using a triple quadrupole tandem mass spectrometer coupled with a high-performance liquid chromatograph. Statistical analyses included univariate tests and univariate and multivariate receiver operating characteristic (ROC) curves. RESULTS Of the 188 metabolites, 131 were determined in the samples. Additionally, 48 sums and ratios of metabolites were calculated. Significant differences (P<0.05) were found for the 6 metabolites of sphingolipids and glycerophospholipids and ratio serotonin synthesis. Multivariate ROC curve analysis was performed for phosphatidylcholine 40: 4 and serotonin synthesis ratio, which were selected based on the univariate ROC curve analysis results. This combined indicator's area under the curve (AUC) demonstrated greater sensitivity and specificity than the individual variables. CONCLUSIONS Our study sheds new light on differences in serum metabolite concentrations between patients under 5 years of age with asthma and IgE-dependent allergy and patients without asthma or IgE-dependent allergy. A multiclassifier integrating PC 40: 4 and serotonin synthesis ratio is a promising biomarker for diagnosing childhood asthma with IgE-dependent allergy, facilitating faster, more precise, and personalized diagnosis and treatment of pediatric patients.
BACKGROUND Due to their good differentiation abilities, human periodontal ligament stem cells (hPDLSCs) and tissue engineering treatment modalities are used to treat bone defects due to periodontitis. This in-vitro study aimed to determine the effects of different melatonin concentrations on the viability of cultured periodontal ligament stem cells. MATERIAL AND METHODS Cultured hPLDSCs were treated with different melatonin concentrations. The study groups were divided into 9 concentration-based groups: Group 1, untreated hPDLSCs; Group 2, hPDLSCs exposed to physiological doses of melatonin (1 μM); Group 3, hPDLSCs exposed to 10 μM melatonin; Group 4, hPDLSCs exposed to 20 μM melatonin; Group 5, hPDLSCs exposed to 30 μM melatonin; Group 6, hPDLSCs exposed to 40 μM melatonin; Group 7, hPDLSCs exposed to 50 μM melatonin; Group 8, hPDLSCs exposed to 100 μM melatonin; Group 9, hPDLSCs exposed to 150 μM melatonin. Cell viability was evaluated at 14 and 21 days using the AlamarBlue (AB) Cell Viability Assay, a toxicity assay. RESULTS Melatonin had a positive effect on hPDLSC viability in all test groups. A general pattern of decreasing cell viability with increasing melatonin concentrations was observed, with Groups 8 and 9 showing the lowest viability. An abrupt pattern was detected in Groups 4 and 7, where a sudden, significant increase in cell viability was observed, in contrast with the general observed pattern. CONCLUSIONS In conclusion, the hPDLSCs remained viable when exposed to the melatonin doses used, under osteogenic media, except for the 100µM and 150 µM melatonin groups. Therefore, further studies are required with hPDLSCs exposed to different concentrations of melatonin.
