An immunofluorescence analysis of TUBB3 expression was performed in tissue samples from patients with stage I and stage III non-small cell lung cancer (NSCLC, n = 65). A TUBB3 level of 50
A computational study of six-membered radical cations of 1,4-thioxane and thiomorpholine using density functional theory methods (DFT and TD DFT) shows that the relatively high thermodynamic stability of the “chair” conformation and the asymmetric distribution of spin and charge density may explain the absence of thermal transformations up to the matrix melting temperature and photochemical transformations in the visible region for these species, in contrast to the related radical cations of 1,4-dithiane and 1,4-dioxane. The experimental data confirm the stabilization of the aforementioned species in low-temperature freon matrices in the “chair” conformation and the absence of thermal or photochemical transformations in the temperature range of 77–135 K.
Deep eutectic solvents (DESs) used to synthesize functional materials, such as oxide ceramics and carbon nanomaterials, are reviewed. It is shown that DESs allow for lower synthesis temperatures and control of particle morphology. Key challenges of these methods together with the advantages are systematized: residual impurities, difficulties to control stoichiometry, and high viscosity. The review is supplemented by the analysis of global patent activity and the achievements of Russian scientific schools in this field. Further studies to optimize purification and to improve reproducibility are needed for the widespread adoption of these technologies.
Rational design of disulfide bonds in P. verruculosum polysaccharide monooxygenase was carried out. It was shown that the increase in enzyme activity and thermostability correlates with the location of the disulfide bond in the region of a highly mobile loop near the active center in the three-dimensional structure of the enzyme. It was also demonstrated that thermal denaturation of P. verruculosum polysaccharide monooxygenase is reversible and the enzyme fully restores activity after incubation at 4°C.
Neurodegenerative diseases, characterized by irreversible decline in cognitive function, attract significant attention from researchers. Based on their symptomatic and pathophysiological features, these diseases are classified into several main groups, with Alzheimer’s, Parkinson’s, and Huntington’s diseases being the most prevalent. Since the key effector (initiator) of the pathological process remains unknown for most of these conditions, multiple alternative hypotheses attempt to explain their mechanisms. This review presents the infectious origin hypothesis of neurodegenerative diseases and the current state of supporting evidence. The strengths and weaknesses of this hypothesis were discussed, and based on the proposed etiology, existing therapeutic approaches were outlined, along with potential methods for their improvement. For instance, the use of broad-spectrum antimicrobial agents demonstrated low efficacy, highlighting the need for the development of more targeted, specialized, and advanced treatments.
