In order to investigate the significance of the pairing of Radix Bupleurum(RB)and Radix Paeoniae Alba(RP),the optimal extraction conditions were determined by single-factor investigation,and the volatile components of RB,RP and RB-RP were determined by headspace solid-phase microextraction(HS-SPME)coupled with gas chromatography-mass spec-trometry(GC-MS),and the percentage content of each component was determined by area normalization and subjected to principal component analysis(PCA).The results showed that there were ten volatile components shared by RB,RP and RB-RP,but the contents of the shared components were different.The PCA scores of the three pairs were significantly different,with the highest PCA score for the volatile components of the pair.Most of the volatile components of the pair of RB-RP came from RB,and the combination of RB-RP produced new active ingredients,and from the volatility point of view,the combina-tion of RB-RP has certain potency and toxicity reduction effects.
In order to promote the efficient enrichment of huperzine A,a new co-culture system(CCS),utilizing the symbiotic relationship between Huperzia serrata and endophytic fungi,was proposed.The optimal culture conditions of the CCS were ob-tained based on the cell survival rate,callus induction rate and multiplication rate.Huperzine A and ergosterol were applied as indicators to control the proportion of botanical cells and endophytic fungi.The growth balance between botanical cells and en-dophytic fungi was maintained for the optimal time of subculturing.The results showed that the best culture conditions for CCS was:100 Lux Luminous level,95％humidity,MS liquid medium,20.0 g/L sucrose,5.0 mg/L 2,4-dichlorophenoxyacetic acid and 1.0 mg/L kinetin.The 4th day was the optimal subculturing time with the highest concentration of huperzine A,which increased by 49.34％compared with the huperzine A level on the 1stday.The growth of endophytic fungal had a posi-tive effect on the enrichment of huperzine A when the concentration ratio of huperzine A to ergosterol was kept between 1.5 and 2.2.This study provides a new proposal to increase the production of huperzine A with high efficiency and low cost,which can effectively solve the problem of insufficient drug source and has a good application prospect.
This study aimed to investigate the variety and distribution of 7H-azulene[1,2,3-i,j]isoquinoline-7-one(7H-AI7O)components from Acorus traditional Chinese medicine.Using liquid-liquid extraction,solvent extraction,column chro-matography separation technology and thin-layer chromatography,the enrichment fragments of 7H-AI7O components from Acori Calami Rhizome and Acori Tatarinowii Rhizome were prepared with neo-tatarine as the reference substance,and then UPLC-Q-TOF/MS2 was used to analyze the composition of 7H-AI7O.Identified by thin-layer chromatography,among 10 bat-ches of Acorus traditional Chinese medicine,only batches S2,S4 and S5 of Acori Calami Rhizome and S6 of Acori Tatarinowii Rhizome contained 7H-AI7O components.According to the analysis of the mass spectrometry fragmentation rule of neo-tata-rine and target compounds,five 7H-AI7O compounds were detected in batch S2,two 7H-AI7O compounds were detected in batch S4,and only neo-tatarine was detected in batch S5 and S6.Although the number of test samples is limited,according to the analysis of the current results,there are 7H-AI7O components in both Acori Calami Rhizome and Acori Tatarinowii Rhizo-me,the distribution of the origin of Acori Calami Rhizome containing 7H-AI7O compounds is wider than that of Acori Tatari-nowii Rhizome,and types of 7H-AI7O components in Acori Calami Rhizome were more than in Acori Tatarinowii Rhizome.This study provides experimental basis for multiple plant sources,trace component detection,and related mechanisms of action of 7H-AI7O components.
To study the diversity and difference of rhizosphere microorganisms of Dendrobium nobile with different epiphytic substrates under the same cultivation environment,in order to clarify the influence of substrates on its rhizosphere microorgan-isms.The 16S and ITS sequencing of rhizosphere microorganisms of D.nobile in five epiphytic substrates,including stone and wood,using high flux sequencing technology.The diversity and difference of D.nobile rhizosphere microorganisms were com-pared by combining OTU analysis,Alpha diversity analysis and principal component analysis.A total of 56 phyla,112 classes,275 orders,377 families,and 621 genera of bacteria have been identified;Fungi:13 phyla,47 classes,123 orders,273 fami-lies,503 species.The dominant genus of D.nobile fungi in the stone matrix is unidentified_Cantharellales_Sp bacteria;the dominant bacteria are Sphingomonas,Fecal bacilli,and unidentified_Chloroplast;The dominant fungus genus of D.nobile in wood matrix is unidentified_Cantharellales_Sp bacteria,Entoloma,the dominant bacteria is Faecalibacterium.The relative a-bundance of D.nobile in the stone stroma was significantly different from that in the wood stroma,and the dominant genuses were different.The function prediction analysis showed that the epiphytic matrix was closely related to the function of D.nobile rhizosphere microbial community.The difference of rhizosphere microorganisms in different epiphytic substrates of D.nobile may affect the composition of its mycorrhizal fungi,thereby affecting the formation of quality differences in D.nobile.This study provides an experimental basis for the correlation of"substrate-rhizosphere microorganisms-mycorrhizal fungi"of D.nobile,its growth and development,and the accumulation of secondary metabolites,as well as the basis for"the Dendrobium grown on the stone has the superior quality"of D.nobile and the reasonable selection of cultivation substrate of D.nobile.
This study aims to study the constituents from the pericarp of Tamarindus indica Linn.and their antioxidant activi-ty.Eighteen compounds were isolated from the acetone extract by chromatographic separation technology.On the basis of NMR spectroscopic data,they were identifeid as luteolin(1),7,3',4'-trihydroxyflavone(2),apigenin(3),7,4'-dihydroxyflavone(4),geraldone(5),astragalin(6),quercetin(7),kaempferol(8),acacetin(9),eriodictyol(10),naringenin(11),butin(12),dihydrokaempferol(13),3',5,5',7-tetrahydroxyflavanone(14),5,6,7,4'-tetrahydroxyflavanone(15),3,3',5,5',7-pentahydroxyflavanone(16),catechin(17),epicatechin(18).Ten of them(2,4-6,9,11,13-16)were isolated from this ge-nus for the first time.By ORAC assay in vitro,it was found that four extracts and nine compounds(1,2,7-10,12,13,17)ex-hibited antioxidant activity.
