ARCaDia-selected short DNA core positions a latent fosylate-type warhead for protein-gated covalent capture.
NMR structures of free and protein-bound aggregation-induced-emission (AIE) fluorophores conjugated to a peptide explain the fluorescence increase upon binding.
Leveraging the intrinsic reactivity of cysteine, isoTOP-ABPP (activity-based protein profiling) has been a powerful chemoproteomic platform for cysteine quantification, ligand, and chemical modification discovery.
By integrating FRET-based competition with OBOC screening, this platform directly selects compounds that disrupt PPIs rather than merely bind targets, enabling the discovery of potent, functional PPI inhibitors.
Small interfering RNAs (siRNAs) were prepared with vinyl nucleobases and imperfect duplex reaction sites that enable selective inverse electron-demand Diels-Alder (IEDDA) reactions with tetrazines in living cells. These bioorthogonal reactions...
