Use of microbiological researches is an integral part of experimental researches during working out of structure, technology, an estimation of quality of new medicinal forms with antibacterial substances. Applied microbiological methods (in an agar and membrane filtrations) in the course of experimental researches have allowed to carry out a choice of the optimum polymeric basis, to prove concentration of metronida-zole, to study spectrum and force of antibacterial activity, to track microbiological cleanliness of the developed new structures of biosoluble films.