This article shows the modern views of the resistance of microorganisms to disinfectants and mechanisms of its formation. Different versions of the impact of subbactericidal concentrations of disinfectants, which can be replicated in laboratory conditions. The results of experiments on the formation of induced acquired resistance by the test strain of E.coli to disinfectants from the group of quaternary ammonium compounds (QAC) in the laboratory. The estimation of changes in the microbiological properties, sensitivity to antibiotics and disinfectants, performed electronic microscopy of bacteria in the process of resistance appearance. Any evidence of the possibility of microbial resistance to QAC-containing disinfectants under the influence of repeated impacts of subbactercidal concentrations in laboratory conditions, the time frame of its appearance.