Response of ammonia emissions to litter moisture content was investigated under laboratory-controlled conditions. A dynamic, flow-through chamber system was designed to test ammonia emissions from broiler litter samples under various litter moisture contents. It was observed that ammonia emissions are very sensitive to litter moisture content. As water was added to the litter, the NH3-N content in the litter increased. However, measurements of ammonia concentrations in the chamber and total nitrogen loss from litter all suggested that water applied to the litter actually suppressed ammonia emissions for a short time. After enough time ( one week or so) was allowed, higher moisture content in litter can eventually result in higher ammonia emissions. It was also noticed that, at very high litter moisture content, even when extra time was allowed, ammonia concentrations began to decrease as moisture content further increased.