Cloning and Functional Characterization of AhyAP-T65Lig, an ATP-Dependent DNA Ligase from Trabzonvirus AP-T65

DNA ligases are essential enzymes in all living organisms and viruses. They play critical roles in genome maintenance and recombination by sealing single-strand nicks and repairing damaged DNA. Due to these features, DNA ligases are widely used in gene cloning applications and in vitro processing of DNA. In this study, the DNA ligase–encoding gene from Trabzonvirus AP-T65 was cloned and sequenced. AP-T65 DNA ligase (AhyAP-T65Lig) shared 51.81