Interest in electrical lysis of biological cells on a microfluidic chip device has increased as it allows for the rapid recovery of intracellular contents without introducing lytic agents. In this research, a new microfabricated device for cell lysis has been developed. The device utilized a microfabricated interdigital electrode array to generate a pulsed electric field with a low voltage, a requirement for effective cell lysis. It is therefore particularly suitable in applications of miniature bio-analysis systems such as lab-on-a-chip systems, where cell lysis is required to obtain the intracellular contents for further analysis. C2C12 cells have been tested using the microfabricated device and the lysis of C2C12 cell was observed using a microscope. The factors which could affect the cell lysis efficiency were investigated such as voltage and pulse duration. The results obtained demonstrated that C2C12 cells could be effectively lysed by the developed device. Key words: Cell lysis, pulsed electric field, microfabricated device, C2C12.
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