Figure S14 from Palmitic Acid Accumulation Activates Fibroblasts and Promotes Matrix Stiffness in Colorectal Cancer

Figure S14: PA promotes mCAF activation and regulates cytokine secretion via the NF-κB pathway. (A) Immunofluorescence staining detection of mCAFs in subcutaneous and orthotopic CRC models of C57BL/6 and Apcmin/+ mice, green: FN1, red: α-SMA (Scale bars = 1mm and 50μm). (B) FN1-positive α-SMA cell ratio in tumors of C57BL/6, BALB/c, and Apcmin/+ mice. (C) CCK-8 assay determine the optimal inhibitory concentration of the NF-κB pathway inhibitor QNZ in CRC cells. (D) QNZ inhibition of PA-induced CSF-1, TGF-β1, and CXCL8 secretion in CRC cells. Statistical significance was determined by unpaired Student t test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, no significance.