Immobilization of Laccase on Magnetic PEGDA–CS Inverse Opal Hydrogel for Enhancement of Bisphenol A Degradation in Aqueous Solution

Endocrine disruptors such as bisphenol A(BPA)adversely affect the environment and hu-man health.Laccases are used for the efficient biodegradation of various persistent or-ganic pollutants in an environmentally safe manner.However,the direct application of free laccases is generally hindered by short enzyme lifetimes,non-reusability,and the high cost of a single use.In this study,laccases were immobilized on a novel magnetic three-dimensional poly(ethylene glycol)diacrylate(PEGDA)-chitosan(CS)inverse opal hydrogel(LAC@MPEGDA@CS@IOH).The immobilized laccase showed significant improvement in the BPA degradation performance and superior storage stability compared with the free lac-case.91.1％of 100 mg/L BPA was removed by the LAC@MPEGDA@CS@IOH in 3 hr,whereas only 50.6％of BPA was removed by the same amount of the free laccase.Compared with the laccase,the outstanding BPA degradation efficiency of the LAC@MPEGDA@CS@IOH was maintained over a wider range of pH values and temperatures.Moreover,its relative activity of was maintained at 70.4％after 10 cycles,and the system performed well in actual water matrices.This efficient method for preparing immobilized laccases is simple and green,and it can be used to further develop ecofriendly biocatalysts to remove organic pollutants from wastewater.