■ ■ Provides a single separation technique and a single instrumentation approach for rapid lipids analyses IN T RO DU C T IO N The analysis of complex lipids has historically been a challenging task and may require a variety of analytical techniques. Lipids are generally recognized as hydrophobic compounds, but the properties of complex lipids containing phosphorus, sulfur, sugar, and nitrogen have a wide polarity range. Developing separation-based methodology utilizing a single technique is recognized as a common challenge for scientists researching lipidomic and metabolomic applications.