Light sheet microscopy has become a popular imaging modality for multi-dimensional imaging. Its unconventional orthogonal setup between the excitation and emission axis allows for light to optically section the sample reducing the amount of background signal when compared to epifluorescence and reducing the laser power deposited into the sample when compared to confocal imaging. As the optics are different from a traditional inverted microscope, traditional mounting methods cannot be used. Instead, different mounting methods were created to work around the setup and adapt to the sample.
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