MP46-18 ANTIBIOTIC-RESISTANT UROPATHOGENS FORM BIOFILMS PREDOMINANTLY ON DISTAL AND LUMINAL ASPECTS OF CATHETERS AND ARE INHIBITED BY A NOVEL CETYLPYRIDINIUM CHLORIDE-BASED FORMULATION

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder I (MP46)1 Apr 2020MP46-18 ANTIBIOTIC-RESISTANT UROPATHOGENS FORM BIOFILMS PREDOMINANTLY ON DISTAL AND LUMINAL ASPECTS OF CATHETERS AND ARE INHIBITED BY A NOVEL CETYLPYRIDINIUM CHLORIDE-BASED FORMULATION Glenn Werneburg*, Nadine Henderson, Raymond Rackley, Anh Nguyen, Daniel Shoskes, Amanda Le Sueur, Anthony Corcoran, Aaron Katz, Jason Kim, Annie Rohan, and David Thanassi Glenn Werneburg*Glenn Werneburg* More articles by this author , Nadine HendersonNadine Henderson More articles by this author , Raymond RackleyRaymond Rackley More articles by this author , Anh NguyenAnh Nguyen More articles by this author , Daniel ShoskesDaniel Shoskes More articles by this author , Amanda Le SueurAmanda Le Sueur More articles by this author , Anthony CorcoranAnthony Corcoran More articles by this author , Aaron KatzAaron Katz More articles by this author , Jason KimJason Kim More articles by this author , Annie RohanAnnie Rohan More articles by this author , and David ThanassiDavid Thanassi More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000901.018AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Urinary catheter biofilms promote bacterial resistance to antibiotics and host cell defenses. Biofilm formation is critical for the pathogenesis of catheter-associated UTI. Here we sought to determine the bacterial composition and antibiotic resistance patterns of bacterial biofilms, as well as their locations on catheters over time. Further, we aimed to test a novel antiseptic formulation, containing cetylpyridinium chloride (CPC) as the active product ingredient combined with pro-adherence factors, for growth inhibition of the biofilm bacterial isolates in liquid medium. METHODS: Urinary catheters were collected following removal from patients, were sectioned and stained for biofilms using crystal violet, and spectrophotometry was used for quantitation. Viability was confirmed via growth in liquid culture, and next generation sequencing via the MicroGenDX platform was employed. Five isolates were cultured, normalized, and subcultured in the presence of the 1:10 or 1:100 antiseptic formulation, or a normal saline control, and growth was measured with spectrophotometry. RESULTS: Thirty-three urinary catheters were included in the study. Biofilm formation increased as a function of time up to 5 weeks. Biofilms predominated on the luminal, balloon, and distal (p=0.034 vs. proximal end) portions of catheters. Uropathogenic bacteria were detected in 10 of 10 samples analyzed using next-generation sequencing (Figure 1a). Genes conferring bacterial resistance to multiple antibiotics were detected in isolates. Relative to the normal saline control, the CPC formulation consistently inhibited growth of the bacterial isolates in a dose-dependent manner (Figure 1b). CONCLUSIONS: Biofilms were composed of uropathogenic bacteria, which were often resistant to common antibiotics. A novel antiseptic formulation inhibited growth of biofilm isolates in a dose-response manner. Biofilm reduction techniques including routine antiseptic irrigation warrant further investigation, as they are well-positioned to target the luminal-predominant biofilms identified in our study and thus may reduce the risk of CAUTI. Source of Funding: NIH T32GM008444 and F30AI112252 (GTW), R01GM062987 (DGT), AACN-Sigma Theta Tau Critical Care Award (AJR) © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e680-e681 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Glenn Werneburg* More articles by this author Nadine Henderson More articles by this author Raymond Rackley More articles by this author Anh Nguyen More articles by this author Daniel Shoskes More articles by this author Amanda Le Sueur More articles by this author Anthony Corcoran More articles by this author Aaron Katz More articles by this author Jason Kim More articles by this author Annie Rohan More articles by this author David Thanassi More articles by this author Expand All Advertisement PDF downloadLoading ...