Recently it has been the main trend for the application of polymer materials in the interdisciplinary tissue engineering area. Polymer materials have shown their versatility and played main roles in constructing tissue engineering scaffolds. This book chapter briefly reviews the materials requirements for tissue engineering application, and focus on polymer materials including both natural and synthetic polymers. The frequently used and newly developed fabrication technologies for constructing tissue engineering scaffolds are described. Some surface modification/functionalization approaches to the tissue engineering scaffolds are also discussed. Recent advances for the development of polymer scaffolds in skin, cartilage, blood vessel, and nerve tissue engineering are also summarized.