Synthetic Seeds in Black Huckleberry: Clonal Fidelity and Oxidative Balance Assessment

Ensuring genetic fidelity and proper antioxidant regulation is essential for synthetic seed technology and conservation of economically important plant species. In this study, we evaluated the effects of plant growth regulators (PGRs) and storage temperature on the germination and regeneration of encapsulated somatic embryos of Vaccinium membranaceum, while verifying genetic fidelity and physiological competence. The highest somatic embryogenesis was obtained from leaf explants cultured on berry basal medium (BM) containing 4.6 µM thidiazuron (TDZ) and 1.13 µM 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos were encapsulated in 3