Transcriptome Profiling of Silique Lncrnas Reveals Novel Insights into Silique Length in Radish (raphanus Sativus L.)

Radish is a widely cultivated vegetable with known medicinal value and produces siliques that differ significantly in number and size. The present study compares normal Duanye-13 radish (CK-type) siliques with mutant radish siliques induced by chemical mutagenesis at the young (YS), short (SS), and long (LS) silique stages. Differences in long noncoding ribonucleic acid (lncRNA) expression were investigated using high-throughput RNA sequencing and bioinformatic analyses, revealing 45 324 new lncRNAs in radish siliques. lncRNA expression was higher in YS and LS mutant lines than in CK-type lines, and expression in SS mutants tended to be lower than in CK-type lines. Furthermore, lncRNA expression at the LS stage tended to be lower than at the YS stage. A Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the target genes revealed that the most significantly different pathways were oxidative phosphorylation, base excision repair, and linoleic acid metabolism. The present study lays the foundations for future investigations into the mechanisms by which lncRNAs regulate radish seed formation and clarifies their potential role in radish breeding and biological resource development.