Objective To analyse the human leukocyte antigen(HLA)-A, B, DRB1 allele and haplotype frequencies in Jiangxi Chinese Han unrelated bone marrow donors.Methods Frequencies of HLA-A,B,DRB1 allele and haplotype were calculated by maximum likelihood estimator method,which based on the genotype data of 2 210 Jiangxi Han unrelated bone marrow donors.Results A total of 72 HLA alleles were observed in Jiangxi Han bone marrow donors and the most frequent were A*02, A*11, A*24, A*33, B60, B*46, B*13, B*58, B*51, B75,B62,DRB1*09,DRB1*12, DRB1*15, DRB1*04, DRB1*08, DRB1*11, DRB1*14 and DRB1*13. Among 358 kinds of A-B haplotype, there were 26 kinds included A2-B46, A11-B60 and A33-B58 with frequency higher than 0.01,and 68 kinds with statistically significant and positive linkage disequilibrium. Moreover, among 420 kinds of B-DR haplotype, there were 22 haplotypes included B46-DR9, B13-DR15 and B75-DR12 with frequency higher than 0.01,and 82 haplotypes with statistically significant and positive linkage disequilibrium. Only 386 kinds of A-B-DR haplotype were informative with frequency higher than or equal to 4.52 per 10 000 in 2 518 haplotypes, and a total of 39 kinds of A-B-DR haplotype included A2-B46-DR9, A2-B46-DR8, A11-B13-DR15, A33-B58-DR13,A33-B58-DR17 and A30-B13-DR7 were the most frequent with frequency higher than 0.005.Conclusion Distribution of HLA-A,B,DRB1 alleles and haplotypes in Jiangxi Chinese Han is relatively closed to Southern Chinese Han population and also showed its own characterization. The frequencies of HLA allele and haplotype from this study may be served as the reference standard for Jiangxi Chinese Han.

Objective To analyse the distributive characteristic of human leukocyte antigen(HLA) -A, B, DRB1 genes and haplotypes for Anhui Chinese Han population. Methods Frequencies of HLA-A,B,DRB1 genes and haplotypes which based on 2 816 unrelated bone marrow donors from Anhui were calculated by square root method and maximum likelihood estimator method respectively, and then compared with other population.Results A total of 78 HLA alleles or antigens were observed and the most frequent were A~*02, A~*11, A~*24, A~*33, A~*30, B~*13, B~*46, B60, B62, B~*58, B61, DRB1~*09, DRB1~*15, DRB1~*04, DRB1~*07, DRB1~*12, DRB1~*08, DRB1~*13, DRB1~*11 and DRB1~*14 in Anhui Chinese Han population. There were 26 kinds of haplotypes included A2-B46 and A30-B13 with frequency higher than 0.01 among 440 kinds of A-B haplotypes;whereas 27 kinds of haplotypes included B13-DR7 and B46-DR9 among 481 kinds of B-DR haplotypes. Additionally, at least 80 kinds of A-B haplotypes and 102 kinds of B-DR haplotypes were statistically significant and positive linkage disequilibrium respectively, and among them,there were 17 kinds of strong linkage disequilibrium A-B haplotypes mainly included A2-B46, A30-B13, A33-B58, A33-B44 and A2-B38, whereas, 18 kinds of strong linkage disequilibrium B-DR haplotypes mainly included B13-DR7, B46-DR9, B58-DR17, B44-DR13, B54-DR4, B52-DR15, B58-DR3 and B57-DR7. The numbers of haplotypes with frequency higher than or equal to 3.55 per 10 000 were 537 in 3 488 kinds of A-B-DR haplotypes, and 27 kinds of haplotypes included A30-B13-DR7 and A2-B46-DR9 were the most common haplotypes with frequency higher than 0.005.Conclusion Distribution of HLA-A, B,DRB1 genes and haplotypes in Anhui Chinese Han population is relatively closed to Northern Chinese Han population, but impressed by Southern Chinese Han to a certain extent.

为正确保存造血干细胞捐献者的HLA-A、B和DRB1基因型信息等数据资料,提高患者寻找HLA配合供者的机会,本所2000年受中国红十字会总会委托,与深圳市海纳信息技术有限公司合作,研制开发了"中国造血干细胞捐献者资料库深圳地区电脑网络管理系统",该系统将生物技术与信息技术相结合,通过局域网络的构建和应用,实现了深圳地区造血干细胞捐献者资料库的组织、征集、登记、DNA抽提、HLA基因分型,以及HLA患者检索服务的电子信息化管理,已为中国造血干细胞捐献者资料库接受、推广和应用.