【Objective】 To investigate the correlation between TCF7L2 gene polymorphism and type 2 diabetes mellitus in Chinese Han population from Huaihai region. 【Methods】 The TCF7L2 gene polymorphisms of 200 type 2 diabetes mellitus patients (case group) and 200 healthy people (control group) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). 【Results】 TCF7L2 rs12255372 genotype frequency and allele frequency revealed that there were no significant difference between type 2 diabetes and health people (P 0.05). Logistic regression analysis, regardless on single factor or multifactors such as: age, sex and blood pressure, did not suggest that the various genotypes of TCF7L2 rs12255372 were risk factors of type 2 dibetes (P 0.05). 【Conclusions】 No relevance between the gene polymorphisms of TCF7L2 rs12255372 and T2DM in Chinese Han population from Huaihai region has been observed.