One strain of Newcastle disease virus(NDV) was isolated from diseased pheasant in a pheasant farm in Guangxi in 2008,and the biological characteristic of the strain was determined.The results showed that the mean death time of chicken embryos for the isolated strain was 50.4 hours,and the intracerebral pathogenicity index to one-day-old chicks was 15.8.The homology of functional domains of F gene nucleotide sequence of the isolated strain with standard strains F48E9 and Lasota was 84.5% and 81.8%,respectively.The deduced amino acids sequence of cleavage site was 112G-R-R-Q-K-R-F117.These results showed the isolated strain was a strong virulent strain of NDV.

With 10-days goose embryo,a strain of virus was isolated from the spleen and liver of 5 days old gosling died from gosling plague in Guangxi.It was determined by the real-time quantitative PCR method.The length of 589 nucleotides of VP3 were amplified,then the fragments were cloned and sequenced.Analysing the sequences of VP3 gene of the isolated strain,GDFSH strain,82-0308 strain,HLJ01 strain and HBZF07 strain,their homologies of nucleotides were 96.2% to 98%.The results showed that the isolated strain was goose parvovirus.