Beer waste yeast protein and ethylene glycol were mixed at different ratios and hot press-molded to produce beer waste yeast protein plastic. The effects of ethylene glycol on the structure and property of beer waste yeast protein plastic were studied by material testing machine, integrated thermal analyzer, Fourier transform infrared spectrometer and scanning electron microscopy. The results show that plastic with ethylene glycol mass fraction of 35% had a maximum elongation. The water absorption of the plastic increased with increase of ethylene glycol content. The hydrogen bonds between the ethylene glycol and the beer waste yeast protein wcakend the hydrogen bonds among the protein molecules and enlarged the elongation of plastic. The ethylene glycol reuld improve the microstructure and toughness of beer waste yeast protein plastic.
[Objective]The aim was to study the best extration method and technology of protein from waste beer yeast.[Method]Four extraction methods were adopted in this research,which were ultrasonic extraction,freeze-thaw extraction,salt extraction and alkali extraction.[Result]Under the best extraction process and conditions,the protein extraction rate of ultrasonic extraction,freeze-thaw extraction,salt extraction and alkali extraction were 3.47%,4.15%,5.66% and 22.81% respectively.The best extraction technology of protein from beer waste yeast was alkali extraction,the best conditions of alkali extraction were 3∶10 of yeast to liquid ratio,1% of NaOH concentration,1 h of extraction time and 60 ℃ of extracting temperature.[Conclusion]It might provide the theoretic and experimental foundation for the extraction of protein from beer waste yeast in large scale.
Based on extensive literature search,species,components,pharmacology and clinical application of Androsace L. were summarized,which will provide reference basis for the exploration and utilization.