Objective To investigate the effects of HepG2 and HepG2.2.15 cells steatosis on the mRNA and protein expressions of suppressors of cytokine signaling-3(SOCS-3) and sterol regulatory element binding proteins (SREBP-1c).Methods The cell model of chronic hepatitis B (CHB) combined with nonalcoholic fatty liver disease (NAFLD) was successfully constructed using an oleic acid-induced HepG2 and HepG2.2.15 cells steatosis.Cells were divided into HepG2 cell control group (HepG2 cell control group), HepG2.2.15 cell control group (HepG2.2.15 cell control group), HepG2 cell steatosis group (HepG2 cell steatosis group) and HepG2.2.15 cell steatosis group (HepG2.2.15 cell steatosis group).The expression levels of SOCS-3 and SREBP-1c mRNA were detected by real-time quantitative polymerase chain reaction (PCR).Changes in protein expressions of SOCS-3 and SREBP-1c were measured by western blot.Results SOCS-3 mRNA expression level in HepG2.2.15 cell control group was significantly lower than that in HepG2 cell control group (P<0.01).The level in HepG2 cell steatosis group was also significantly lower than that in HepG2 cell control group (P<0.01).However, the level of SOCS-3 mRNA in HepG2.2.15 cell steatosis group was lower than HepG2.2.15 cell control group with no statistical significance (P=0.173).There was interaction between cells and steatosis (F=25.547, P<0.01).The expression of SREBP-1c mRNA in HepG2.2.15 cell control group was significantly lower than that in HepG2 cell control group (P<0.01), and was significantly higher in HepG2.2.15 cell steatosis group than that in HepG2.2.15 cell control group (P<0.01).There was no significant difference between HepG2 cell steatosis group and HepG2 cell control group (P=1.000).There was interaction between cells and steatosis (F=5.04, P<0.05).Western blot analysis showed that protein levels of SOCS-3 and SREBP-1c in steatosis cells at 48 h and 72 h were significantly higher than those in non-alcoholic steatosis cells.Conclusions Protein expressions of SOCS-3 and SREBP-1c are up-regulated in both steatosis groups.Factorial analysis shows that there is interaction between cells and steatosis.HBV gene could inhibit SOCS-3 mRNA expression and promote the expression of SREBP-1c mRNA in steatosis cells.

近年来,我国医疗卫生事业迅速发展,医院建设取得了长足的进步,硬件设施、医疗技术水平、医院管理水平以及服务能力均显著提升。综合医院要在市场经济环境中持续发展,必须依靠科技进步和创新来提高医院的综合竞争力,必须依赖于医学科研的发展,科研的发展离不开科研管理工作,科研管理工作决定于好的科研管理队伍。但是,目前医院科研管理队伍的建设远远落后于医院发展,本研究就综合医院科研管理队伍的建设问题进行了分析与探讨。

Objective To explore the relationship between the polymorphism of C-509T at the promoter region of transforming growth factor(TGF)-β1 gene and non-alcoholic fatty liver disease(NAFLD).Methods A polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was established to detect TGF-β1 genotypes.Results ①There were no significant differences in the frequencies of TGF-β1-509C/T gene between the patients and control group(P>0.05).②The CT genotype prevailed in both the SFL and the NASH patients.The frequency of CC genotype(27.4%) was higher than that of TT genotype(19.2%) in NASH patients while the frequency of TT genotype(21.6%) was higher than that of CC genotype(4.1%) in SFL patients.There were significant differences between all the groups(P<0.05).Conclusion No evident association was found between TGF-β1-509C/T polymorphism and NAFLD,but the presence of Callete at position-509 may play an important role in the progression of NAFLD.