目的:观察hepcidin对小鼠糖代谢及运动行为能力的影响.方法:小鼠随机分为空白对照组、铁负荷组(2mg、1mg、0.5mg)、hepcidin(5 ug)+铁负荷组(2mg、1mg、0.5mg)；血细胞计数仪检测血液中血红蛋白含量,全自动生化分析仪检测血清铁含量,试剂盒检测血清中转铁蛋白(Tf)和总铁结合力(TIBC)；葡糖糖氧化酶法测定血糖；Morris水迷宫实验观察小鼠学习记忆能力的的改变.结果:与空白对照组比较,铁负荷组小鼠血清Fe,Hb明显增加,Tf和TIBC明显减少,血糖升高,且呈剂量依赖性；Morris水迷宫实验结果显示,铁负荷组小鼠与空白对照组比较,隐匿平台时间明显增加,而靶象限活动时间明显下降；而hepcidin组均出现相反方向变化.结论:铁过负荷可干扰小鼠糖代谢,升高血糖,并引起小鼠认知行为和空间记忆力下降；而hepcidin可抑制铁负荷引起的血糖升高和学习记忆能力变化.

Objective:To observe the effect of hepcidin on deposit of mice brain iron and the exercise and behavior ability of mice,and discuss the mechanism.Methods:An equal number of male and female mice was randomly divided into seven groups:control goup,overload-iron group(2 mg.ml-1,1 mg.ml-1,0.5 mg.ml-1)and hepcidin group(5μg)+ overload-iron group(2mg.ml-1,1mg.ml-1,0.5mg.ml-1).Intramuscular injection of iron dext-ran of different concentrations was given every other day for eight weeks to establish an iron-overload animal model.Blood hemoglobin content was tested by blood count instrument,serum iron content was tested by automatic biochemical analyzer,serum transferrin(Tf)and total iron binding capacity(TIBC)were detected using kit.The iron content of brain tissue was measured by atomic absorption spectrometry,and the ROS of brain tissue was measured by ROS kit.The Morris water maze tests were done respectively to observe the change of mice′s movement behavior.Results:Compared with the control group,serum Fe and Hb increased significantly,Tf and TIBC significantly reduced,Fe content in these brain areas including cerebellum,midbrain and striatum and ROS increased significantly in a dose-dependent manner.Morris water maze test results showed that,compared with control group,a significant increase in the average escape time and a significant decrease in target quadrant activity time were observed in overload-iron group.But the opposite results were observed in hepcidin+ overload-iron group.Conclusion:Iron overload of mice cerebellum,midbrain and striatum cause obvious toxic damage,leading to brain oxidative stress,cognitive behavior and spatial memory decline,but all these effects can be inhibited by hepcidin.