Aim.The investigation of response of S. cerevisiae cells to the effect of ozone in different doses and determination of total antioxidant capacity (TAC) of these cells.Methods.TAC was estimated with the chemiluminescent method when introducing ozone into S. cerevisiae cells.The damage of cells was determined by the methods of fluorescent microscopy and flow cytometry using fluorescent dye Square-460.Results.It was shown that under the ozone dosed administration into the S. cerevisiae cells suspension the chemiluminescence flash occurred in response to each new ozone doze.The flash amplitude slightly changes under low doses and decreases after achieving some total ozone dose, being characteristic for these cells.At the same time the damaged cells appear, the number of which augments with increasing the administered ozone dose.Conclusions.We assessed the TAC of S. cerevisiae cells, as the ozone dose, which may be neutralized by the systems of their antioxidant protection: 240 ± 20 nmol/10 6 cells.The S. cerevisiae cells are resistant to the effect of excessive ozone until its doses exceed the TAC value for these cells.

Partial ejaculatory duct obstruction, due to either a congenital or an acquired cyst or ejaculatory duct stenosis secondary to calcification, chronic inflammation, can produce a wide spectrum of seminal fluid abnormalities. Sperm density may range from azoospermia to normospermia while ejaculate volume can be low to normal. Sperm motility is consistently diminished (less than 30%). We have treated 2 patients with ejaculatory duct stenosis whose diagnosis was accurately made with transrectal ultrasonography (TRUS). We now suggest that TRUS be used when there is a low semen volume (less than 1.0 cc), or low motility (less than 30%), or oligospermia (less than 20 million sperm/mL), and normal findings on physical examination with normal serum gonadotropin values in the absence of any other explanation.

It was shown that preliminary exposure of a solvent (water) to low-intensity laser radiation reduces the tryptophan fluorescence intensity, and this fluorescence quenching effect is retained throughout the temperature range explored (from 8 up to 50 degrees C). The effects found are interpreted as resulting from changes in solvent properties induced by the action of electromagnetic radiation on interaction of water molecules with solute.

Experiments on young puberal Wistar rats have shown that chronic effect of the electrostatic field brings lipid peroxidation (LPO) to a new level of the dynamic equilibrium. Metabolism of lipids proved to be sensitive to this factor. But the systems regulating LPO actively holds a new equilibrium state. Inert LPO products of the lipopigments type can bind vitamin E whose content in tissues remains unchanged.

Experiments on young puberal Wistar rats have shown that chronic effect of the electrostatic field brings lipid peroxidation (LPO) to a new level of the dynamic equilibrium. Metabolism of lipids proved to be sensitive to this factor. But the systems regulating LPO actively holds a new equilibrium state. Inert LPO products of the lipopigments type can bind vitamin E whose content in tissues remains unchanged.

The action of electrostatic field (320 kV/m) on the adaptability has been studied in experiments on young Wistar rats (females). Some parameters of carbohydrate, lipid and protein metabolism, and the process of lipid peroxidation were observed in blood serum, liver and brain. Glucose content was observed to increase in all the tissues, liver glycogen, cholesterol/phospholipid ratio. The urea and malondialdehyde increased in the liver and brain. The experiments have shown that the physiological adaptability is high.