The traditional SQL injection attacks are used to attack RFID system,so user data is in a state of insecurity in the back-end database.In this paper we first analyzes the implementation process of the SQL injection attack on RFID back-end database,then proposed a hash lock-based anti-SQL injection method to solve the security problem,last gave the simulation experiments results to show the method can effectively prevent RFID SQL injection attacks.

This study is designed to investigate the effects of ginsenoside Rg1 on murine peritoneal macrophages in vitro.The single cell suspension of murine peritoneal macrophages was prepared under sterile condition.After pre-incubated by Rg1 for 4 hours,the cells were stimulated with LPS at a final concentration of 10 μg/ml for 24 hours,then 1 μm diameter of the Yellow-Green Beads(1×1010/L) was added and the phagocytosis ability of macrophages was detected by flow cytometry(FCM).At the same time,the amount of NO produced by macrophages was detected by Griess kit,the ROS production by macrophages was assayed by H2DCFDA staining,the intracellular calcium concentration in macrophages was deteced by Fluo-4/AM staining.SytoxR Green plus Fluorescence Microplate Rader were used to detect the cells survive condition.The results showed Rg1 could inhibit the phagocytosis ability of macrophage stimulated with or without LPS.It also indicated that Rg1 could inhibit the production of NO and ROS both in the presence and absence of LPS.In addition,Rg1 could reduce the apoptosis of peritoneal macrophages induced by ION.Furthermore,Rg1 could inhibit the cell apoptosis induced by CHX and CTX.All these prompt Rg1 has an significant anti-inflammatory effects on macrophages,and can reduce the apoptosis induced by different stimuli,which provides a basis for the further development of Rg1 as an immune therapy drug.

Aim:To investigate the effect of Chuankezhi(CKZ) on apoptosis,NO release and cytokine secretion of murine peritoneal macrophages in vitro,and the mechanism of its action. Methods: The single cell suspension of murine peritoneal macrophages was prepared under sterile condition.After preincubated with CKZ for 4 hours,H2O2、CHX、CTX were added to induce cell apoptosis.24 hours later,sytox green plus fluorescence microplate rader was used to detect apoptosis of the cells.After pre-incubated with CKZ for 4 hours,the cells were stimulated with LPS at a final concentration of 10 10μg/mL and IFN-γ at a final concentration of 80ng/mL.24 hours later,the amount of NO produced in macrophages was detected by Griess kit,and release of cytokines was detected by cytometric bead array(CBA) plus flow cytometry.Results: CKZ could inhibit cell apoptosis induced by H2O2、CHX and CTX.It also indicated that CKZ could upregulate NO production and multiple cytekine secretion by resting macrophages and downregulate NO release and cytokine synthesis on stimulated macrophages.Conclusion:CKZ could inhibit cell apoptosis.It also had dual-directional modulation of NO secretion and IL-6、IL-10、MCP-1、IFN-γ、TNF-α production.

Objective To study the mechanisms of Chinese herbal drug,chuangkezhi(CKZ)on the dual directional modulation of T-lymphocyte activation in mice.Methods The mouse lymph node was separated in sterilization and the single lymphocyte suspension was prepared.Radix Morindae Officinalis extract(50 μg·mL-1),Epimedium brevicornum Maxim extract(100 μg·mL-1)and CKZ(50 μg·mL-1 Radix Morindae Officinalis + 100 μg·mL-1 Epimedium brevicornum Maxim)were added separately.After pre-incubation for 4 h,the stimulator was added.12 h later,the flow cytometry in combination with double-color fluorescence antibody staining technology were used to detect the expression of CD69,the early activated marker of CD3+T lymphocyte.Results Compared with blank control group,in Morindae Officinalis extract treatment group,CD69 expression in CD3+T lymphocyte was upregulated apparently.Compared with stimulation group,Morindae Officinalis extract co-stimulated CD69 expression.Epimedium brevicornum Maxim extract did not impact apparently CD69 expression in CD3+T lymphocyte at the resting state,but it inhibited CD69 expression of CD3+T lymphocyte induced by the stimulator.As compared with the two extracts,CKZ indicated obvious dual immune modulation,meaning that it upregulated CD69 expression of CD3+T lymphocyte at resting state and downregulated CD69 expression of CD3+T lymphocyte at stimulating state.Conclusion The dual-directional modulation of CKZ on CD3+T lymphocytes activation Results from the cooperation of Morindae Officinalis and Epimedium brevicornum Maxim,in which Morindae Officinalis promotes immune response and Epimedium brevicornum Maxim inhibits immune response.