银屑病是以遗传为基础的多因素影响并诱发的慢性增生性皮肤病,难以根治,临床多种治疗方法都只能控制病情.如果皮损面积不超过其体表面积的25%,可不考虑内服药物治疗, 使用外用药物即可达到比较理想的目的;另外,外用药物使用面积小,吸收少,药物不良反应较少,不容易引起肝、肾功能损害,比较安全[1].头部银屑病在临床上常见,因皮损暴露,给患者身心造成的不良影响较大;患者多蓄发遮掩,但使用外用药治疗时因头部覆以毛发使其剂型选择受到限制;加之以往大部分外用药成分以糖皮质激素为主,疗效欠佳且停药后极易出现皮损反跳及加重现象[2],故头部银屑病较顽固,治疗效果不佳.我院自2009年8月-2011年2月,采用卡泊三醇搽剂联合温泉浴、松馏油软膏封包治疗头部银屑病82例,取得显著疗效.现报告如下.

Objective To construct a phage displayed human single-chain antibody library derived from patients with psoriasis and screen the specific single-chain antibody against human tumor necrosis factor α(hTNFα).Methods The serum samples from 33 patients with psoriasis were analyzed for IgG against hTNFα by indirect ELISA.The pComb3X_scFv library was constructed by phage displayed technique using the separated peripheral blood lymphocytes(PBLs),and identified for capacity,accuracy and diversity.Microtiter plate was coated with hTNFα,based on which the solid phase biopanning of specific hTNFα antibody was performed by 4 cycles ofadsorption-elution-amplification.Results The anti-hTNFα IgG level in sera of patients with psoriasis was significantly higher than that in healthy individuals(P 0.01).A phage displayed human single-chain antibody library with a capacity of about 4 × 106,a recombination rate of more than 90% and good diversity was successfully constructed using the gene materials of the specific antibody.Specific phage displayed antibody was enriched by biopanning of hTNFα.Conclusion Specific single-chain antibody against hTNFα was successfully screened by using the constructed phage displayed human single-chain antibody library derived from patients with psoriasis.

我科2006年11月至2008年4月采用高能辐射治疗机治疗18例阴囊湿疹患者,疗效显著,现报告如下.